Clinical significance of circulating tumor cells detected by an invasion assay in peripheral blood of patients with ovarian cancer.
Clinical significance of circulating tumor cells detected by an invasion assay in peripheral blood of patients with ovarian cancer.
复制标题
DOI：
10.1016/j.ygyno.2008.09.021
复制
发表时间：
2009-01
影响因子：
4.7
通讯作者：
Pearl, Michael L.
中科院分区：
文献类型：
--
作者：
Fan, Tina;Zhao, Qiang;Chen, John J.;Chen, Wen-Tien;Pearl, Michael L.
关键词：
循环肿瘤细胞（CTC）的侵袭性生长促进癌症转移。本研究的目的是评估侵袭性CTC与疾病分期、CA-125水平和患者生存率的相关性，侵袭性CTC通过一种新的细胞侵袭测定法检测。使用细胞侵袭试验评估来自71名接受卵巢恶性肿瘤评估的患者的外周血样品中侵袭性CTC的存在，所述细胞侵袭试验用细胞粘附基质（CAM）富集并鉴定肿瘤细胞。侵袭性CTC被鉴定为表现出CAM侵袭（CAM+）并表达标准上皮标志物（Epi+）的细胞。43例（60.6%）患者可检测到CTC：0/5例良性患者，1/10例（10%）早期患者，39/52例（73.1%）晚期患者和3/4例（75%）未分期患者（p值< 0.001）。CTC计数范围为0-149 CTC/ml，III/IV期患者表现出显著高于I/II期患者（6.0 CTC/ml）和良性患者（0 CTC/ml，p值= 0.001）的平均计数（41.3 CTC/ml）。观察到CTC计数和CA-125水平之间呈正相关（斯皮尔曼相关系数r = 0.309，p值= 0.035）。Kaplan-Meier曲线显示可检测到CTC的患者的无病生存期显著降低（中位生存期15.0个月vs. 35.0个月，对数秩p值= 0.042）。肿瘤分级和肿瘤组织学不影响CTC检测。浸润性CTC可以在大多数上皮性卵巢癌患者中检测到，并且可以预测较短的无病生存期。此外，较高的CTC计数可能反映了晚期疾病和较高的CA-125水平。
The invasive growth of circulating tumor cells (CTCs) propagates cancer metastasis. The aims of this study were to evaluate the association of invasive CTCs, detected by a novel cell invasion assay, with disease stage, CA-125 level and patient survival. Peripheral blood samples from 71 patients undergoing evaluation for ovarian malignancy were assessed for the presence of invasive CTCs using a cell invasion assay that enriches and identifies tumor cells with a cell adhesion matrix (CAM). Invasive CTCs were identified as cells exhibiting CAM invasion (CAM+) and expressing standard epithelial markers (Epi+). 43 (60.6%) patients had detectable CTCs: 0/5 benign patients, 1/10 (10%) early stage, 39/52 (73.1%) late stage and 3/4 (75%) unstaged patients (p-value < 0.001). CTC counts ranged from 0-149 CTCs/ml with stage III/IV patients exhibiting significantly higher mean counts (41.3 CTCs/ml) than stage I/II patients (6.0 CTCs/ml) and benign patients (0 CTCs/ml, p-value = 0.001). A positive correlation between CTC count and CA-125 level was observed (Spearman correlation coefficient r = 0.309, p-value = 0.035). Kaplan-Meier curves revealed a significant decrease in disease-free survival in patients with detectable CTCs (median survival 15.0 months vs. 35.0 months, log-rank p-value = 0.042). Tumor grade and tumor histology did not influence CTC detection. Invasive CTCs can be detected in a majority of epithelial ovarian cancer patients and may predict shorter disease-free survival. Furthermore, higher CTC counts may reflect later stage disease and higher CA-125 levels.
登录
查看更多内容
DOI：
10.2165/00066982-200005010-00009
发表时间：
2000-03-01
期刊：
MOLECULAR DIAGNOSIS
影响因子：
--
作者：
Crisan, D;Ruark, DS;Dicarlo, RG
通讯作者：
Dicarlo, RG
影响因子：
45.3
作者：
Braun, S;Schindlbeck, C;Pantel, K
通讯作者：
Pantel, K
影响因子：
4.7
作者：
CAIN, JM;ELLIS, GK;LIVINGSTON, RB
通讯作者：
LIVINGSTON, RB
影响因子：
4.7
作者：
Judson, PL;Geller, MA;Carson, LF
通讯作者：
Carson, LF
影响因子：
11.2
作者：
Ghersi, Giulio;Zhao, Qiang;Chen, Wen-Tien
通讯作者：
Chen, Wen-Tien