Polysaccharide biosynthesis-related genes explain phenotype-genotype correlation of Microcystis colonies in Meiliang Bay of Lake Taihu, China.
Polysaccharide biosynthesis-related genes explain phenotype-genotype correlation of Microcystis colonies in Meiliang Bay of Lake Taihu, China.
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多糖生物合成相关基因解释太湖梅梁湾微囊藻菌落表型-基因型相关性
DOI：
10.1038/srep35551
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发表时间：
2016-10-18
影响因子：
4.6
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Xu S;Sun Q;Zhou X;Tan X;Xiao M;Zhu W;Li M
分析了2014年太湖微囊藻的16S rDNA、16S-23S rDNA-ITS、cpcBA-IGS、mcygene和几个多糖生物合成相关基因(EPSL和TagH)，并对其形态进行了鉴定。我们的结果表明，无论是形态还是分子工具，包括16S rDNA、16S-23S ITS和cpcBA-IGS都不能区分有毒和无毒的微囊藻。16S、16S-23S和cpcBA-IGS检测结果表明，微囊藻的表型与其基因型之间没有明显的相关性，但与多糖生物合成相关的基因可以区分微囊藻的表型。此外，对2015年收集的微囊藻多糖生物合成相关基因(EspLandTagH)的序列进行了分析。样品以M.成鱼率(60-100%)和微囊藻。用pLandTagH将60%~100%的韦森伯格氏吸虫分为不同的支系。因此，确认了m。我们的名字是M。成鱼可以通过与多糖生物合成相关的基因(EspLandTagH)来区分。本研究对填补微囊藻分子生物学分类与形态分类学之间的空白具有重要意义。
The 16S rDNA, 16S-23S rDNA-ITS,cpcBA-IGS,mcygene and several polysaccharide biosynthesis-related genes (epsL andTagH) were analyzed along with the identification of the morphology ofMicrocystiscolonies collected in Lake Taihu in 2014.M. wesenbergiicolonies could be distinguished directly from other colonies usingespL.TagHdivided all of the samples into two clusters but failed to distinguish different phenotypes. Our results indicated that neither morphology nor molecular tools including 16S rDNA, 16S-23S ITS andcpcBA-IGS could distinguish toxic and non-toxic species among the identifiedMicrocystisspecies. No obvious relationship was detected between the phenotypes ofMicrocystisand their genotypes using 16S, 16S-23S andcpcBA-IGS, but polysaccharide biosynthesis-related genes may distinguish theMicrocystisphenotypes. Furthermore, the sequences of the polysaccharide biosynthesis-related genes (espLandTagH) extracted fromMicrocystisscums collected throughout 2015 was analyzed. Samples dominated byM. ichthyoblabe(60–100%) andM. wesenbergii(60–100%) were divided into different clade by bothespLandTagH, respectively. Therefore, it was confirmed thatM. wesenbergiiandM. ichthyoblabecould be distinguished by the polysaccharide biosynthesis-related genes (espLandTagH). This study is of great significance in filling the gap between classification of molecular biology and the morphological taxonomy ofMicrocystis.
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影响因子：
2.1
作者：
Otsuka, S;Suda, S;Watanabe, MM
通讯作者：
Watanabe, MM
影响因子：
4.5
作者：
Fujimoto, N;Sudo, R;Inamori, Y
通讯作者：
Inamori, Y
影响因子：
2.8
作者：
Haande, Sigrid;Ballot, Andreas;Edvardsen, Bente
通讯作者：
Edvardsen, Bente
影响因子：
2.9
作者：
Mazur-Marzec, Hanna;Browarczyk-Matusiak, Grazyna;Plinski, Marcin
通讯作者：
Plinski, Marcin
影响因子：
2.1
作者：
Imai, Hiroyuki;Chang, Kwang-Hyeon;Nakano, Shin-ichi
通讯作者：
Nakano, Shin-ichi