Inhibitory effect of mouse neocortex layer I on the underlying cellular network
Inhibitory effect of mouse neocortex layer I on the underlying cellular network
复制标题
小鼠新皮质 I 层对底层细胞网络的抑制作用
DOI：
--
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发表时间：
2003
影响因子：
3.4
通讯作者：
Y. Amitai
中科院分区：
文献类型：
--
作者：
D. Shlosberg;S. L. Patrick;Y. Buskila;Y. Amitai
哺乳动物皮层I层是皮层下和皮层内起源的轴突以及锥体神经元的顶端树突簇的汇聚点。第一层的一个突出特征是抑制性轴突的广泛丛，它起源于所有皮质层的星状细胞。这种抑制投射在皮层网络活动中的作用尚未确定。我们研究了第一层内的抑制输入对底层细胞网络活动的影响程度。在II/III层记录场电位（FPs）。在记录移液管上方的第1层集中应用GABAA阻断剂微毒素，或去除第1层，可在控制等强度的刺激下产生更大的FP振幅。当抑制被部分阻断时，去除第1层导致产生癫痫样事件所需的阈值刺激强度显著降低，并且这些事件的传播速度上升。中间神经元化学标记的免疫细胞化学证明，第一层的抑制性输入主要是生长抑素免疫反应（SM - ir），因此第一层包含皮层中大约三分之一的SM - ir轴突。Calretinin免疫反应性轴突也以较低的密度出现在第一层。我们得出结论，第一层对皮层细胞网络的影响包括一个显著的抑制成分。这种抑制具有适度的抑制作用，其去除会增加皮层回路的兴奋性，但不足以诱发癫痫现象。
The mammalian cortical layer I is a convergence site for axons of sub‐ and intracortical origin, and the apical dendritic tufts of pyramidal neurons. A prominent feature of layer I is an extensive plexus of inhibitory axons, which originate from stellate cells in all cortical laminae. The role of this inhibitory projection in the activity of cortical networks has yet to be determined. We investigated the degree to which inhibitory inputs within layer I affect the activity of the underlying cellular network. Field potentials (FPs) were recorded in layer II/III. Focal application of the GABAA blocker picrotoxin in layer I above the recording pipette or the removal of layer I resulted in larger FP amplitudes for stimulations at control‐equal intensities. When inhibition was partially blocked, the removal of layer I caused a significant reduction in the threshold stimulus intensity required for generating epileptiform events, and a rise in the propagation velocity of these events. Immunocytochemistry for chemical markers of interneurons proved that the inhibitory input to layer I is predominantly somatostatin immunoreactive (SM‐ir), such that layer I contains approximately one‐third of all SM‐ir axons in the cortex. Calretinin‐immunoreactive axons were also present in layer I at a lower density. We conclude that the impact of layer I on the cortical cellular network includes a significant inhibitory component. This inhibition confers a moderate restraining influence, and its removal increases the excitability of cortical circuits, but not sufficiently to induce epileptic phenomena.
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影响因子：
2.5
作者：
P. Schweitzer;S. Madamba;G. Siggins
通讯作者：
P. Schweitzer;S. Madamba;G. Siggins
成年大鼠体感皮层 GABAA 受体介导的抑制的电生理图。
DOI：
10.1152/jn.1996.75.4.1589
发表时间：
1996
期刊：
Journal of neurophysiology.
影响因子：
--
作者：
Salin,PA;Prince,DA
通讯作者：
Prince,DA
影响因子：
3.7
作者：
Gonchar, Y;Burkhalter, A
通讯作者：
Burkhalter, A
影响因子：
2.5
作者：
CHAGNACAMITAI, Y;CONNORS, BW
通讯作者：
CONNORS, BW
影响因子：
3.7
作者：
Geula, C;Mesulam, MM
通讯作者：
Mesulam, MM