Cloning southern corn rust resistant gene RppK and its cognate gene AvrRppK from Puccinia polysora.

Cloning southern corn rust resistant gene RppK and its cognate gene AvrRppK from Puccinia polysora.
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DOI:
10.1038/s41467-022-32026-4
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发表时间:
2022-07-29
影响因子:
16.6
通讯作者:
--
中科院分区:
综合性期刊1区
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广谱抗性在作物育种中具有重要价值。然而,其机制在很大程度上是未知的。本文报道了玉米抗南方玉米锈病(SCR) NLR基因RppK及其同源Avr基因AvrRppK的克隆,该基因来自玉米锈病的病原菌多孢锈病(Puccinia polysora)。AvrRppK基因在所有检测的分离株中没有序列变异。它在感染过程中高表达,可抑制模式触发免疫(PTI)。此外,RppK渗入到玉米自交系和杂交种中,增强了对多聚玉米疫病的抗性,从而在SCR存在的情况下提高了产量。总之,我们发现RppK参与了对多孢霉分离株的抗性,并且可以识别AvrRppK, AvrRppK在多孢霉分离株中广泛分布并保守。南方玉米锈病(SCR)是由多孢契契菌(Puccinia polysora)引起的主要玉米病害,可造成严重的产量损失。在这里,作者报道了一种CC-NB-LRR型R基因RppK的表达导致玉米易感品系对SCR产生抗性,并且它可以识别多角菌产生的效应物AvrRppK。
Broad-spectrum resistance has great values for crop breeding. However, its mechanisms are largely unknown. Here, we report the cloning of a maize NLR gene, RppK, for resistance against southern corn rust (SCR) and its cognate Avr gene, AvrRppK, from Puccinia polysora (the causal pathogen of SCR). The AvrRppK gene has no sequence variation in all examined isolates. It has high expression level during infection and can suppress pattern-triggered immunity (PTI). Further, the introgression of RppK into maize inbred lines and hybrids enhances resistance against multiple isolates of P. polysora, thereby increasing yield in the presence of SCR. Together, we show that RppK is involved in resistance against multiple P. polysora isolates and it can recognize AvrRppK, which is broadly distributed and conserved in P. polysora isolates. Southern corn rust (SCR) caused by Puccinia polysora is a major maize disease that can result in major yield loss. Here, the authors report the expression of a CC-NB-LRR type of R gene RppK results in SCR resistance in susceptible maize lines and it can recognize the effector AvrRppK produced by P. polysora.
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