Identification and verification of genes related to pollen development and male sterility induced by high temperature in the thermo-sensitive genic male sterile wheat line
Identification and verification of genes related to pollen development and male sterility induced by high temperature in the thermo-sensitive genic male sterile wheat line
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温敏基因雄性不育系小麦花粉发育及高温诱导雄性不育相关基因的鉴定与验证
DOI：
10.1007/s00425-021-03601-8
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发表时间：
2021-03
期刊：
影响因子：
4.3
通讯作者：
Song Xiyue
中科院分区：
文献类型：
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作者：
Yang Xuetong;Ye Jiali;Niu Fuqiang;Feng Yi;Song Xiyue
主要结论生物信息学分析确定了小麦育性调控基因的功能。大麦条纹花叶病毒诱导的基因沉默证实了TaMut11和TaSF3基因参与花粉发育并与育性转换有关。因此，研究花粉发育和雄性不育相关基因的功能具有重要的意义，而这些基因的功能目前还不完全清楚。本研究对小麦温敏核不育新品系偃湛4110S及其近等基因系偃湛4110进行了分析。通过比较转录组基础生物信息学和加权基因共表达网络进一步筛选出部分中枢基因，在盐湛4110 S中鉴定出与花粉发育和高温诱导雄性不育相关的基因TaMut11和TaSF3。通过大麦条纹花叶病毒诱导的基因沉默进一步验证，TaMut11和TaSF3基因的沉默导致花粉败育，最终导致育性下降。这些结果为环境敏感型核不育小麦的败育机理提供了依据。
Main conclusionBioinformatic analysis identified the function of genes regulating wheat fertility. Barley stripe mosaic virus-induced gene silencing verified that the genesTaMut11andTaSF3are involved in pollen development and related to fertility conversion.AbstractEnvironment-sensitive genic male sterility is of vital importance to hybrid vigor in crop production and breeding. Therefore, it is meaningful to study the function of the genes related to pollen development and male sterility, which is still not fully understand currently. In this study, YanZhan 4110S, a new thermo-sensitive genic male sterility wheat line, and its near-isogenic line YanZhan 4110 were analyzed. Through comparative transcriptome basic bioinformatics and weighted gene co-expression network to further identify some hub genes, the genesTaMut11andTaSF3associated with pollen development and male sterility induced by high-temperature were identified in YanZhan 4110S. Further verification through barley stripe mosaic virus-induced gene silencing elucidated that the silencing ofTaMut11andTaSF3caused pollen abortion, finally resulting in the declination of fertility. These findings provided data on the abortive mechanism in environment-sensitive genic male sterility wheat.
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细胞学和转录组分析揭示了与同源四倍体水稻花粉败育相关的减数分裂和糖代谢基因的突然表达
影响因子：
3.1
作者：
Chen, Lin;Shahid, Muhammad Qasim;Liu, Xiangdong
通讯作者：
Liu, Xiangdong
影响因子：
1.6
作者：
Cakir, Cahid;Scofield, Steven R.
通讯作者：
Scofield, Steven R.
番茄果实中病毒诱导的基因沉默。
影响因子：
7.2
作者：
Fu, DQ;Zhu, BZ;Luo, YB
通讯作者：
Luo, YB
影响因子：
7.4
作者：
Valentine, T;Shaw, J;Lacomme, C
通讯作者：
Lacomme, C
DOI：
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发表时间：
1996
期刊：
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影响因子：
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作者：
通讯作者：
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