RNA Interference-Mediated Knockdown of Genes Encoding Spore Wall Proteins Confers Protection against Nosema ceranae Infection in the European Honey Bee, Apis mellifera.
RNA Interference-Mediated Knockdown of Genes Encoding Spore Wall Proteins Confers Protection against Nosema ceranae Infection in the European Honey Bee, Apis mellifera.
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RNA 干扰介导的孢子壁蛋白编码基因敲低可保护欧洲蜜蜂 (Apis mellifera) 免受蜜蜂微孢子虫感染
DOI：
10.3390/microorganisms9030505
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发表时间：
2021-02-27
期刊：
影响因子：
4.5
通讯作者：
Huang SK
中科院分区：
文献类型：
--
作者：
He N;Zhang Y;Duan XL;Li JH;Huang WF;Evans JD;DeGrandi-Hoffman G;Chen YP;Huang SK
小孢子虫是欧洲蜜蜂（Apis mellifera）的一种细胞内寄生虫，引起严重的小孢子虫病，已与世界范围内的蜜蜂群体损失有关。诺塞马病唯一登记的治疗方法是富马吉林-b，这引起了对耐药性和脱靶效应的担忧。在许多国家，特别是在欧洲，Fumagillin-B被禁止在蜜蜂群中使用。因此，迫切需要新的有效的治疗方案来治疗蜜蜂小虫病。基于RNA干扰（RNAi）的方法可能是控制蜜蜂疾病的有效策略。我们利用基于rnai的方法探索了沉默两个蜜蜂孢子壁蛋白（SWP）基因序列的治疗潜力。我们的研究表明，单独或联合口服SWP8和SWP12对应的dsRNAs可显著降低感染N. ceranae的蜜蜂的孢子载量，提高免疫力，延长寿命。本研究的结果增强了我们对蜜蜂宿主对微孢子虫感染的反应的理解，并强调了基于rnai的治疗方法是一种很有希望的治疗蜜蜂疾病的方法。
Nosema ceranae (Opisthosporidia: Microsporidia) is an emergent intracellular parasite of the European honey bee (Apis mellifera) and causes serious Nosema disease which has been associated with worldwide honey bee colony losses. The only registered treatment for Nosema disease is fumagillin-b, and this has raised concerns about resistance and off-target effects. Fumagillin-B is banned from use in honey bee colonies in many countries, particularly in Europe. As a result, there is an urgent need for new and effective therapeutic options to treat Nosema disease in honey bees. An RNA interference (RNAi)-based approach can be a potent strategy for controlling diseases in honey bees. We explored the therapeutic potential of silencing the sequences of two N. ceranae encoded spore wall protein (SWP) genes by means of the RNAi-based methodology. Our study revealed that the oral ingestion of dsRNAs corresponding to SWP8 and SWP12 used separately or in combination could lead to a significant reduction in spore load, improve immunity, and extend the lifespan of N. ceranae-infected bees. The results from the work completed here enhance our understanding of honey bee host responses to microsporidia infection and highlight that RNAi-based therapeutics are a promising treatment for honey bee diseases.
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影响因子：
3.4
作者：
Chen, Yue-Wen;Chung, Wei-Ping;Huang, Wei-Fone
通讯作者：
Huang, Wei-Fone
DOI：
10.1111/1365-2664.12112
发表时间：
2013-08
期刊：
The Journal of applied ecology
影响因子：
--
作者：
Becher MA;Osborne JL;Thorbek P;Kennedy PJ;Grimm V
通讯作者：
Grimm V
影响因子：
3.4
作者：
Alaux, Cedric;Folschweiller, Morgane;Le Conte, Yves
通讯作者：
Le Conte, Yves
影响因子：
3.7
作者：
Borges, Daniel;Guzman-Novoa, Ernesto;Goodwin, Paul H.
通讯作者：
Goodwin, Paul H.
影响因子：
4.6
作者：
Buczek, Katarzyna;Trytek, Mariusz;Tchorzewski, Marek
通讯作者：
Tchorzewski, Marek