Glow flow ionization mass spectrometry of small molecules. A comparison of a glow flow ionization source ('GlowFlow') with electrospray ionization and atmospheric pressure chemical ionization.
Glow flow ionization mass spectrometry of small molecules. A comparison of a glow flow ionization source ('GlowFlow') with electrospray ionization and atmospheric pressure chemical ionization.
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小分子的发光流动电离质谱。 Glow流动电源（“ GlowFlow”）与电喷雾电离和大气压化学电离的比较。
DOI：
10.1002/rcm.9327
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发表时间：
2022-08-15
影响因子：
2
通讯作者：
Brenton, A. Gareth
中科院分区：
文献类型：
--
作者：
Owen, Rhodri N.;Bajic, Stevan;Kelly, Steven L.;Morris, Michael R.;Brenton, A. Gareth
大气压气体放电电离在质谱分析中应用已久。电感耦合等离子体质谱法是一个范例，并广泛用于元素分析。该技术在有机质谱中的应用较少。我们描述了一个简单的源设计，可以很容易地实现在大多数大气压电离（API）系统，并比较其性能与电喷雾电离（ESI）和大气压化学电离（APCI）。在Xevo G2‐S飞行时间质谱仪上使用内部设计的氦气放电源（称为“GlowFlow”）。使用高效液相色谱仪入口将GlowFlow源转移至兼容的Xevo TQ-S三重四极杆质谱仪。并与沃茨ESI和APCI源进行了比较。GlowFlow在Swansea仪器上的初步结果用于建立背景，包括低分子量聚合物、苯甲酸和肉桂酸的分析。Xevo TQ-S三重四极杆质谱仪的性能比较涉及三种测试混合物。正离子辉光流源的方法检测限（六混合）在0.03至10.00 pg之间，线性响应良好，超过2至4个数量级，R2值> 0.98。GlowFlow电离源提供的信号强度比ESI的大气压气相色谱标准混合物的信号强度大一个数量级，并电离了ESI无法电离的几种化合物。目前的GlowFlow设计相对简单，由于其尺寸小，因此可以改装到大多数API系统中。在正离子模式下，GlowFlow设计的灵敏度通常比ESI低一个数量级，但在负离子模式下灵敏度相似，与APCI相当。
Ionization by atmospheric pressure gas discharge has been employed for a long time in mass spectrometry. Inductively coupled plasma mass spectrometry is an exemplar, and widely used for elemental analysis. The technique has less uptake in organic mass spectrometry. We describe a simple source design that can be readily implemented in most atmospheric pressure ionization (API) systems and compare its performance with that of electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI). An in‐house designed helium gas discharge source (referred to as ‘GlowFlow’) was used on a Xevo G2‐S time‐of‐flight mass spectrometer. The GlowFlow source was transferred to a compatible Xevo TQ‐S triple‐quadrupole mass spectrometer using an ultrahigh‐performance liquid chromatograph inlet. Its performance was compared to that of Waters ESI and APCI sources. Preliminary results of GlowFlow on the Swansea instrument are presented to establish context and include analysis of low‐molecular‐mass polymers, benzoic acid and cinnamic acid. Comparison of performance on the Xevo TQ‐S triple‐quadrupole mass spectrometer involved three test mixtures. The method limits of detection (six‐mix) for positive‐ion GlowFlow source were between 0.03 and 10.00 pg with good linear response over two to four orders of magnitude and values of R 2 > 0.98. The GlowFlow ionization source provided a signal intensity that was an order of magnitude greater than that of ESI for an atmospheric pressure gas chromatography standard mix and ionized several compounds that ESI could not. The current GlowFlow design is relatively simple to retrofit to most API systems due to its small size. The sensitivity of the GlowFlow design is typically an order of magnitude less than that of ESI in positive‐ion mode, but similar in sensitivity in negative‐ion mode and comparable to that of APCI.
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影响因子：
7.4
作者：
Cody, RB;Laramée, JA;Durst, HD
通讯作者：
Durst, HD
DOI：
10.1007/s13361-014-0988-7
发表时间：
2014-11-01
影响因子：
3.2
作者：
Kauppila, Tiina J.;Kersten, Hendrik;Benter, Thorsten
通讯作者：
Benter, Thorsten
DOI：
10.1016/s0168-1176(96)04430-8
发表时间：
1997-02-01
期刊：
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES
影响因子：
--
作者：
Wang, XM;Becker, H;Bohme, DK
通讯作者：
Bohme, DK
影响因子：
7.4
作者：
Lloyd, Julie A.;Harron, Andrew F.;McEwen, Charles N.
通讯作者：
McEwen, Charles N.
影响因子：
2
作者：
Trimpin, Sarah
通讯作者：
Trimpin, Sarah