Genetic basis of resistance to southern corn leaf blight in the maize multi-parent population and diversity panel.
Genetic basis of resistance to southern corn leaf blight in the maize multi-parent population and diversity panel.
复制标题
玉米多亲本群体抗南方玉米叶枯病的遗传基础和多样性小组
DOI：
10.1111/pbi.13967
复制
发表时间：
2023-03
影响因子：
13.8
通讯作者：
Yan, Jianbing
中科院分区：
文献类型：
--
作者：
Chen, Gengshen;Xiao, Yingjie;Dai, Sha;Dai, Zhikang;Wang, Xiaoming;Li, Bailin;Jaqueth, Jennifer S.;Li, Wenqiang;Lai, Zhibing;Ding, Junqiang;Yan, Jianbing
关键词：
玉米大斑病是玉米叶部病害之一，严重威胁着世界各地的玉米生产。鉴定玉米抗SLB的遗传变异对提高玉米产量和品质具有重要意义。在这里，我们使用了一个包含8个重组自交系群体的随机开放亲本关联作图群体和一个由513个具有高密度遗传标记的多样性玉米自交系组成的关联作图面板，以剖析SLB抗性的遗传基础。总体而言，109个数量性状位点（QTL）主要是小或中度加性效应，很少上位性效应。与已报道的QTL相比，我们发现了35个（32.1%）新位点。我们发现，抗性等位基因显着丰富的热带加入和约一半的抗性等位基因的频率下降，在适应过程中，由于农艺性状的选择。位于SLB抗性QTL中的大量注释基因被证明参与植物防御途径。综合全基因组关联研究、转录组分析、重测序和基因编辑等技术，我们将ZmFUT1和MYBR92确定为控制抗异星斑螟主效QTL的推定基因。我们的研究结果提供了一个全面的洞察SLB抗性的遗传基础，并提供抗性位点或基因作为作物遗传改良的直接目标。
Southern corn leaf blight (SLB), caused by the necrotrophic pathogen Cochliobolus heterostrophus, is one of the maize foliar diseases and poses a great threat to corn production around the world. Identification of genetic variations underlying resistance to SLB is of paramount importance to maize yield and quality. Here, we used a random‐open‐parent association mapping population containing eight recombinant inbred line populations and one association mapping panel consisting of 513 diversity maize inbred lines with high‐density genetic markers to dissect the genetic basis of SLB resistance. Overall, 109 quantitative trait loci (QTLs) with predominantly small or moderate additive effects, and little epistatic effects were identified. We found 35 (32.1%) novel loci in comparison with the reported QTLs. We revealed that resistant alleles were significantly enriched in tropical accessions and the frequency of about half of resistant alleles decreased during the adaptation process owing to the selection of agronomic traits. A large number of annotated genes located in the SLB‐resistant QTLs were shown to be involved in plant defence pathways. Integrating genome‐wide association study, transcriptomic profiling, resequencing and gene editing, we identified ZmFUT1 and MYBR92 as the putative genes responsible for the major QTLs for resistance to C. heterostrophus. Our results present a comprehensive insight into the genetic basis of SLB resistance and provide resistant loci or genes as direct targets for crop genetic improvement.
登录
查看更多内容
影响因子：
30.3
作者：
Li B;Meng X;Shan L;He P
通讯作者：
He P
影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
影响因子：
12.3
作者：
Liu, Shengxue;Li, Cuiping;Qin, Feng
通讯作者：
Qin, Feng
影响因子：
7.4
作者：
Luo, Hongli;Laluk, Kristin;Mengiste, Tesfaye
通讯作者：
Mengiste, Tesfaye
影响因子：
3.7
作者：
Ali F;Pan Q;Chen G;Zahid KR;Yan J
通讯作者：
Yan J