Synthesis of magnetic resonance-, X-ray- and ultrasound-visible alginate microcapsules for immunoisolation and noninvasive imaging of cellular therapeutics.
Synthesis of magnetic resonance-, X-ray- and ultrasound-visible alginate microcapsules for immunoisolation and noninvasive imaging of cellular therapeutics.
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DOI：
10.1038/nprot.2011.352
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发表时间：
2011-07-14
期刊：
影响因子：
14.8
通讯作者：
中科院分区：
文献类型：
--
作者：
细胞疗法有可能治疗或治愈多种疾病。但是，非侵入性细胞跟踪技术是将这种方法转化为临床环境所必需的。该协议详细详细介绍了创建微胶囊，可通过X射线，超声（US）或磁共振（MR）可见，以封装和免疫分散性细胞疗法。通常使用三个步骤将细胞疗法封装在藻酸盐基质中：（i）使用静电发电机将含含细胞的液藻酸盐的液滴挤出，并通过针尖到含有溶解的分离式阳离子盐的溶液中形成固体凝胶; （ii）将所得的胶凝球覆盖，将多阳离子作为交联； （iii）然后将这些复合物在藻酸盐的第二种溶液中孵育，形成由藻酸盐内部和外层组成的可半透明膜。通过将对比度添加到主藻酸盐层中，可以在第一步中可见微胶囊。这种对比剂包括通过1H MR成像（MRI）检测的超顺磁铁氧化铁。硫酸盐或二硫酸盐的放射性药物，可通过X射线方式检测；或通过19F MRI，X射线和US成像进行多模式检测的全氟化合物乳液。整个合成可以在2小时内完成。
Cell therapy has the potential to treat or cure a wide variety of diseases. Non-invasive cell tracking techniques are, however, necessary to translate this approach to the clinical setting. This protocol details methods to create microcapsules that are visible by X-ray, ultrasound (US ) or magnetic resonance (MR) for the encapsulation and immunoisolation of cellular therapeutics. Three steps are generally used to encapsulate cellular therapeutics in an alginate matrix: (i) droplets of cell-containing liquid alginate are extruded, using an electrostatic generator, through a needle tip into a solution containing a dissolved divalent cation salt to form a solid gel; (ii) the resulting gelled spheres are coated with polycations as a cross-linker; and (iii) these complexes are then incubated in a second solution of alginate to form a semipermeable membrane composed of an inner and an outer layer of alginate. The microcapsules can be rendered visible during the first step by adding contrast agents to the primary alginate layer. Such contrast agents include superparamagnetic iron oxide for detection by 1H MR imaging (MRI); the radiopaque agents barium or bismuth sulfate for detection by X-ray modalities; or perfluorocarbon emulsions for multimodal detection by 19F MRI, X-ray and US imaging. The entire synthesis can be completed within 2 h.
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影响因子：
6.2
作者：
Dufrane, Denis;Goebbels, Rose-Marie;Gianello, Pierre
通讯作者：
Gianello, Pierre
影响因子：
19.7
作者：
Gobin, YP;Viñuela, F;Chow, K
通讯作者：
Chow, K
影响因子：
46.9
作者：
Ahrens, ET;Flores, R;Morel, PA
通讯作者：
Morel, PA
影响因子：
6.2
作者：
Omer, A;Duvivier-Kali, V;Weir, GC
通讯作者：
Weir, GC
DOI：
10.1002/jbm.820240803
发表时间：
1990-08-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
JAYAKRISHNAN, A;THANOO, BC;MOHANTY, M
通讯作者：
MOHANTY, M