Development of tight junctions de novo in the mouse early embryo: control of assembly of the tight junction-specific protein, ZO-1.
Development of tight junctions de novo in the mouse early embryo: control of assembly of the tight junction-specific protein, ZO-1.
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DOI：
10.1083/jcb.108.4.1407
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发表时间：
1989-04
期刊：
影响因子：
--
通讯作者：
Stevenson BR
中科院分区：
文献类型：
--
作者：
Fleming TP;McConnell J;Johnson MH;Stevenson BR
在小鼠植入前胚胎的滋养外胚层生物发生过程中，紧密连接的发展已经使用单克隆抗体识别紧密连接特异性外周膜蛋白，ZO- 1。在免疫印迹中，小鼠胚胎ZO-1的分子量（225 kD）相当于小鼠肝脏中的分子量，在四细胞胚胎中几乎检测不到，尽管后期阶段表现出增加的水平。ZO-1首先检测到免疫细胞化学在压实八细胞阶段，一致或后表达的基底外侧细胞粘附和顶端微绒毛极性。最初，ZO-1是作为一系列的点沿着之间的边界的自由和并列的细胞表面在完整的胚胎或细胞对，但随后染色变得更加线性与囊胚滋养外胚层细胞被接壤的连续ZO-1带。在8细胞阶段抑制细胞粘附延迟ZO-1的外观和随机化其表面分布在一个可逆的方式。微丝破坏，但不是微管解聚，产生重大干扰ZO-1分布。ZO- 1从头组装似乎是独立的近端DNA和RNA的合成，但在8细胞阶段，治疗，不阻止细胞间粘附和极化的蛋白质合成的情况下，基本上被抑制。ZO-1的表面组装，但没有粘附和极化，也受到干扰时，单8细胞与单4细胞相结合。结果表明，小鼠胚胎中的紧密连接发育是上皮生物发生中的次要事件，依赖于细胞粘附和细胞骨架活性的正常表达，并且可以被破坏而不干扰稳定极化表型的产生。
Tight junction development during trophectoderm biogenesis in the mouse preimplantation embryo has been examined using monoclonal antibodies recognizing the tight junction-specific peripheral membrane protein, ZO- 1. In immunoblots, mouse embryo ZO-1 had a molecular mass (225 kD) equivalent to that in mouse liver, was barely detectable in four-cell embryos although later stages exhibited increasing levels. ZO-1 was first detected immunocytochemically at the compacting eight-cell stage, coincident with or just after the expression of basolateral cell adhesion and apical microvillous polarity. Initially, ZO-1 was present as a series of spots along the boundary between free and apposed cell surfaces in intact embryos or cell couplets, but subsequently staining became more linear with blastocyst trophectoderm cells being bordered by a continuous ZO-1 belt. Inhibition of cell adhesion at the 8-cell stage delayed ZO-1 appearance and randomized its surface distribution in a reversible manner. Microfilament disruption, but not microtubule depolymerization, produced major disturbances in ZO-1 distribution. ZO- 1 assembly de novo appeared to be independent of proximate DNA and RNA synthesis but was inhibited substantially in the absence of protein synthesis during the eight-cell stage, a treatment that did not prevent intercellular adhesion and polarization. ZO-1 surface assembly, but not adhesion and polarization, was also perturbed when single eight-cells were combined with single four-cells. The results suggest that tight junction development in mouse embryos is a secondary event in epithelial biogenesis, being dependent upon cell adhesion and cytoskeletal activity for normal expression, and can be disrupted without disturbing the generation of a stably polarized phenotype.
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影响因子：
2.4
作者：
CLAUDE, P
通讯作者：
CLAUDE, P
影响因子：
2.7
作者：
KIDDER, GM;MCLACHLIN, JR
通讯作者：
MCLACHLIN, JR
影响因子：
2.7
作者：
MAGNUSON, T;DEMSEY, A;STACKPOLE, CW
通讯作者：
STACKPOLE, CW
DOI：
10.1083/jcb.107.4.1575
发表时间：
1988-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gumbiner B;Stevenson B;Grimaldi A
通讯作者：
Grimaldi A
影响因子：
2.7
作者：
DUCIBELLA, T;ANDERSON, E
通讯作者：
ANDERSON, E