Tension force-induced ATP promotes osteogenesis through P2X7 receptor in osteoblasts.
Tension force-induced ATP promotes osteogenesis through P2X7 receptor in osteoblasts.
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拉伸力诱导的ATP通过成骨细胞中的P2X7受体促进成骨。
DOI：
10.1002/jcb.24863
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发表时间：
2015-01
影响因子：
4
通讯作者：
Shimizu, Noriyoshi
中科院分区：
文献类型：
--
作者：
Kariya, Taro;Tanabe, Natsuko;Shionome, Chieko;Manaka, Soichiro;Kawato, Takayuki;Zhao, Ning;Maeno, Masao;Suzuki, Naoto;Shimizu, Noriyoshi
正畸牙齿移动通过机械刺激引起牙槽骨吸收和形成。施加在牵引侧的力促进骨形成。三磷酸腺苷（ATP）是骨细胞对机械刺激反应的关键介质之一。然而，张力（TF）诱导的ATP对成骨的影响还没有得到充分的理解。因此，我们研究了TF对MC 3 T3-E1细胞中ATP产生和成骨的影响。在存在或不存在P2 X7受体拮抗剂A438079的情况下孵育细胞，然后使用Flexercell Strain Unit 3000用或不用环状TF（6%或18%）刺激最多24小时。与对照组相比，TF显著增加细胞外ATP释放。与18%TF和对照相比，6%TF对ATP释放的影响最大。6%TF诱导Runx 2和Osterix的表达。6%TF还可增加细胞外基质蛋白（ECMPs）的表达、ALP活性和ECM中钙含量。A438079可阻断6%TF对细胞外基质中Runx 2、Osterix和ECMPs表达、ALP活性和钙含量的刺激作用。本研究表明，TF诱导的细胞外ATP在成骨细胞中释放，表明TF诱导的ATP通过成骨细胞中P2 X7受体的自分泌作用促进成骨。J.细胞。116：12-21，2015.© 2014作者。细胞生物化学杂志由Wiley Periodicals，Inc.出版。
Orthodontic tooth movement induces alveolar bone resorption and formation by mechanical stimuli. Force exerted on the traction side promotes bone formation. Adenosine triphosphate (ATP) is one of the key mediators that respond to bone cells by mechanical stimuli. However, the effect of tension force (TF)-induced ATP on osteogenesis is inadequately understood. Accordingly, we investigated the effect of TF on ATP production and osteogenesis in MC3T3-E1 cells. Cells were incubated in the presence or absence of P2X7 receptor antagonist A438079, and then stimulated with or without cyclic TF (6% or 18%) for a maximum of 24 h using Flexercell Strain Unit 3000. TF significantly increased extracellular ATP release compared to control. Six percent TF had maximum effect on ATP release compared to 18% TF and control. Six percent TF induced the expression of Runx2 and Osterix. Six percent TF also increased the expression of extracellular matrix proteins (ECMPs), ALP activity, and the calcium content in ECM. A438079 blocked the stimulatory effect of 6% TF on the expression of Runx2, Osterix and ECMPs, ALP activity, and calcium content in ECM. This study indicated that TF-induced extracellular ATP is released in osteoblasts, suggesting that TF-induced ATP promotes osteogenesis by autocrine action through P2X7 receptor in osteoblasts. J. Cell. Biochem. 116: 12–21, 2015. © 2014 The Authors. Journal of Cellular Biochemistry published by Wiley Periodicals, Inc.
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