Cytokines, Chemokines, and Growth Factors in Banked Human Donor Milk for Preterm Infants.
Cytokines, Chemokines, and Growth Factors in Banked Human Donor Milk for Preterm Infants.
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DOI：
10.1177/0890334414527795
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发表时间：
2014-08
期刊：
影响因子：
--
通讯作者：
Ashmeade T
中科院分区：
文献类型：
--
作者：
Groer M;Duffy A;Morse S;Kane B;Zaritt J;Roberts S;Ashmeade T
最近，用于喂养早产儿的库存捐赠母乳的可获得性有所增加。这些母乳是由经过仔细筛选的哺乳期妇女捐赠给母乳银行的。然后用Holder方法对牛奶进行巴氏杀菌，以去除所有微生物。加工后的牛奶被冷冻、储存并出售给新生儿重症监护病房(NICU)。储存的供者乳的营养生物利用度已被描述，但对细胞因子、趋化因子和生长因子(CCGF)等免疫成分的保存知之甚少。目的是比较储存的供者母乳中的CCGF与母亲自己的母乳(母亲)中的CCGF。每天从45名NICU入院婴儿的母亲身上收集等量(0.5毫升)，这些母亲出生时体重为1500克。在婴儿入住NICU的6周期间或只要母亲为母亲提供母乳，每个母亲每天的所有母乳都会汇集在一起。通过使用磁珠和MAGPIX仪器对CCGF面板进行多路复用，测量每周汇集的牛奶。储存的供者母乳样本(n=25)的处理和测量方法与MOM相同。多重分析表明，在哺乳6周后，储存的供者乳样中CCGF的水平与从母亲那里获得的值相当。这些数据表明，许多重要的CCGF不会被Holder巴氏杀菌法破坏。
There has been a recent increase in availability of banked donor milk for feeding of preterm infants. This milk is pooled from donations to milk banks from carefully screened lactating women. The milk is then pasteurized by the Holder method to remove all microbes. The processed milk is frozen, banked, and sold to neonatal intensive care units (NICUs). The nutrient bioavailability of banked donor milk has been described, but little is known about preservation of immune components such as cytokines, chemokines, and growth factors (CCGF). The objective was to compare CCGF in banked donor milk with mother's own milk (MOM). Aliquots (0.5 mL) were collected daily from MOM pumped by 45 mothers of NICU-admitted infants weighing < 1500 grams at birth. All daily aliquots of each mother's milk were pooled each week during 6 weeks of an infant's NICU stay or for as long as the mother provided MOM. The weekly pooled milk was measured for a panel of CCGF through multiplexing using magnetic beads and a MAGPIX instrument. Banked donor milk samples (n = 25) were handled and measured in the same way as MOM. Multiplex analysis revealed that there were levels of CCGF in banked donor milk samples comparable to values obtained from MOM after 6 weeks of lactation. These data suggest that many important CCGF are not destroyed by Holder pasteurization.
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影响因子：
3.3
作者：
Braga, Lucylea P. M.;Palhares, Durval B.
通讯作者：
Palhares, Durval B.
影响因子：
2.9
作者：
Delfosse, N. M.;Ward, L.;Lagomarcino, A. J.;Auer, C.;Smith, C.;Meinzen-Derr, J.;Valentine, C.;Schibler, K. R.;Morrow, A. L.
通讯作者：
Morrow, A. L.
影响因子：
5.1
作者：
Garofalo, Roberto
通讯作者：
Garofalo, Roberto
影响因子：
6.3
作者：
Bauer, Jacqueline;Gerss, Joachim
通讯作者：
Gerss, Joachim
影响因子：
2.6
作者：
Jegier, Briana J.;Johnson, Tricia J.;Meier, Paula
通讯作者：
Meier, Paula