Clinical significance of elongation factor-1 delta mRNA expression in oesophageal carcinoma.
Clinical significance of elongation factor-1 delta mRNA expression in oesophageal carcinoma.
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DOI：
10.1038/sj.bjc.6601941
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发表时间：
2004-07-19
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
延伸因子-1（EF-1）δ是EF-1的亚基，EF-1是参与mRNA翻译的延伸步骤的蛋白质复合物，并且最近被认为与致癌转化相关。然而，目前还没有关于EF-1 δ mRNA在恶性肿瘤（包括食管癌）中表达的临床意义的信息。因此，我们定量了EF-1 δ在恶性和良性食管组织中的表达，并将这些水平与食管癌的临床病理参数相关联。从52例接受根治性食管切除术的患者中获得癌性和相应非癌性部分的成对食管组织样本。采用实时定量逆转录-聚合酶链反应对EF-1 delta表达进行定量分析。在52例患者中，38例（73%）的癌组织中观察到延伸因子-1 δ mRNA过表达。癌组织中EF-1 δ mRNA的平均表达水平显著高于癌旁组织（P<0.01）。EF-1 δ的高表达与淋巴结转移（P<0.05）和临床分期有关（P<0.05）。此外，EF-1 δ高表达患者的病因特异性生存率明显低于低表达患者（5年病因特异性生存率分别为23%和54%，P<0.05）。本研究结果表明，食管癌组织中EF-1 delta mRNA的表达明显高于非癌组织，且EF-1 delta mRNA的高表达与食管癌患者的淋巴结转移、疾病分期及预后有关。
Elongation factor-1 (EF-1) delta is a subunit of EF-1, which is a protein complex that participates in the elongation step of mRNA translation and has recently been considered to correlate with oncogenic transformation. However, there has been no information regarding the clinical significance of EF-1 delta mRNA expression in malignant tumours, including oesophageal carcinoma. Thus, we quantitated the expression of EF-1 delta in malignant and benign oesophageal tissues and associated these levels with clinicopathologic parameters of oesophageal carcinoma. Paired oesophageal tissue samples from cancerous and corresponding noncancerous parts were obtained from 52 patients who underwent curative oesophagectomy. Quantitative analyses of EF-1 delta expression were performed using real-time quantitative reverse transcription–polymerase chain reaction. Elongation factor-1 delta mRNA overexpression in cancerous tissues compared to normal counterparts was observed in 38 of 52 (73%) patients. The mean expression level of EF-1 delta mRNA in cancerous tissues was significantly higher than that in noncancerous tissues (P<0.01). A higher expression of EF-1 delta was significantly correlated with lymph node metastases (P<0.05) and advanced stages (P<0.05). Furthermore, the cause-specific survival of patients with a higher expression of EF-1 delta was significantly poorer than those with a lower expression (5-year cause-specific survival rates; 23 and 54%, respectively, P<0.05). The results of this study indicated that EF-1 delta mRNA expression was significantly higher in cancerous compared to noncancerous oesophageal tissues, and a higher expression of EF-1 delta mRNA was correlated with lymph node metastases, advanced disease stages and poorer prognosis for patients with oesophageal carcinoma.
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影响因子：
6.2
作者：
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