RNA aptasensor for rapid detection of natively folded type A botulinum neurotoxin.
RNA aptasensor for rapid detection of natively folded type A botulinum neurotoxin.
复制标题
DOI：
10.1016/j.talanta.2013.09.012
复制
发表时间：
2013-12-15
期刊：
影响因子：
6.1
通讯作者：
Cai, Shuowei
中科院分区：
文献类型：
--
作者：
Janardhanan, Pavithra;Mello, Charlene M.;Singh, Bal Ram;Lou, Jianlong;Marks, James D.;Cai, Shuowei
报道了一种基于表面等离子体共振的RNA适体传感器，用于快速检测天然折叠的A型肉毒神经毒素。使用解毒的重组A型肉毒杆菌神经毒素作为替代物，适体传感器在90分钟内检测到活性毒素。在磷酸盐缓冲液、胡萝卜汁和脱脂牛奶中的检出限分别为5.8ng/ml、20.3ng/ml和23.4ng/ml，而在5倍稀释的人血清中的检出限为22.5ng/ml。毒素从不同样品基质中的回收率在91%至116%之间。最重要的是这种适体传感器能够有效区分天然折叠的毒素和变性的无活性毒素，这对于国土安全监视和威胁评估非常重要。适体传感器稳定超过30天，超过400次注射/再生循环。这种适体传感器由于其鲁棒性、稳定性和可重复使用性，为现场监测的活性肉毒杆菌神经毒素的快速检测提供了很大的希望。
A surface plasmon resonance based RNA aptasensor for rapid detection of natively folded type A botulinum neurotoxin is reported. Using detoxified recombinant type A botulinum neurotoxin as the surrogate, the aptasensor detects active toxin within 90 minutes. The detection limit of the aptasensor in phosphate buffered saline, carrot juice, and fat free milk is 5.8 ng/ml, 20.3 ng/ml and 23.4 ng/ml, respectively, while that in 5-fold diluted human serum is 22.5 ng/ml. Recovery of toxin from disparate sample matrices are within 91% to 116%. Most significant is the ability of this aptasensor to effectively differentiate the natively folded toxin from denatured, inactive toxin, which is important for homeland security surveillance and threat assessment. The aptasensor is stable for more than 30 days and over 400 injections/regeneration cycles. Such an aptasensor holds great promise for rapid detection of active botulinum neurotoxin for field surveillance due to its robustness, stability and reusability.
登录
查看更多内容
影响因子：
4.4
作者：
Sharma, SK;Eblen, BS;Whiting, RC
通讯作者：
Whiting, RC
影响因子：
3.7
作者：
Bagramyan K;Barash JR;Arnon SS;Kalkum M
通讯作者：
Kalkum M
DOI：
10.3109/15569549909036019
发表时间：
1999-01-01
期刊：
JOURNAL OF TOXICOLOGY-TOXIN REVIEWS
影响因子：
--
作者：
Li, L;Singh, BR
通讯作者：
Singh, BR
DOI：
10.1016/j.ijfoodmicro.2008.05.012
发表时间：
2008-08-15
影响因子：
5.4
作者：
Rasooly, Reuven;Stanker, Larry H.;Brandon, David L.
通讯作者：
Brandon, David L.
影响因子：
2.8
作者：
Foster, Keith A.
通讯作者：
Foster, Keith A.