Different Localization of ATP Sensitive K+ Channel Subunits in Rat Testis
Different Localization of ATP Sensitive K+ Channel Subunits in Rat Testis
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大鼠睾丸中 ATP 敏感 K 通道亚基的不同定位
DOI：
10.1002/ar.21348
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
H. Abe
中科院分区：
文献类型：
--
作者：
Ming Zhou;Hui;O. Tanaka;M. Sekiguchi;K. Kawahara;H. Abe
ATP 敏感 K+ (KATP) 通道是细胞膜兴奋性与其细胞生物能状态的重要联系。这些通道由成孔亚基和调节亚基组成。本研究重点关注这些亚基在大鼠睾丸中的细胞表达和定位。 RT-PCR分析显示大鼠睾丸含有5个KATP通道亚基：Kir6.1、Kir6.2、SUR1、SUR2A和SUR2B。免疫印迹检测显示大鼠睾丸中有Kir6.1、Kir6.2、SUR2A和SUR2B蛋白的表达。免疫组织化学显示这些KATP通道亚基在生精细胞、支持细胞和间质细胞的不同部位呈阳性，这表明这些亚基在精子发生中发挥着重要作用。通过双重免疫荧光分析确定Kir6.2与SUR2B在精子顶体或头帽中的共定位，表明KATP通道可能是由Kir6.2和SUR2B在精子顶体中形成的。 KATP通道亚基在生精细胞和支持细胞的细胞膜和膜细胞器中的不同定位表明大鼠睾丸中KATP通道的复杂性和多功能性。 Anat Rec，2011。© 2011 Wiley-Liss, Inc.
ATP sensitive K+ (KATP) channels are important linkage of cell membrane excitability to its cellular bioenergetic state. These channels are composed of pore‐forming subunits and regulatory subunits. The present study focused on the cellular expressions and localizations of these subunits in rat testis. RT‐PCR analysis showed that rat testis contained five KATP channel subunits, Kir6.1, Kir6.2, SUR1, SUR2A and SUR2B. Immunoblot assay showed that proteins of Kir6.1, Kir6.2, SUR2A and SUR2B were expressed in rat testis. Immunohistochemistry revealed these KATP channel subunits were positive in different localizations of spermatogenic cells, Sertoli cells and Leydig cells, which implies these subunits playing important roles in spermatogenesis. Co‐localization of Kir6.2 with SUR2B was determined in acrosome or head cap of spermatids by double immunofluorescence analysis by indicating KATP channel might be formed by Kir6.2 and SUR2B in acrosome of spermatids. Different localizations of the KATP channel subunits in the cell membrane and membranous organelles of spermatogenic cells and Sertoli cells indicated the complex and multiple functions of KATP channels in rat testis. Anat Rec, 2011. © 2011 Wiley‐Liss, Inc.
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