Inhibition of Ribosomal RNA Processing 15 Homolog (RRP15) Suppressed Tumor Growth, Invasion and Epithelial to Mesenchymal Transition (EMT) of Colon Cancer.
Inhibition of Ribosomal RNA Processing 15 Homolog (RRP15) Suppressed Tumor Growth, Invasion and Epithelial to Mesenchymal Transition (EMT) of Colon Cancer.
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DOI：
10.3390/ijms24043528
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发表时间：
2023-02-09
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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作者：
尽管核糖体RNA加工15同源物（RRP 15）与多种癌症的发生有关，并被认为是癌症治疗的潜在靶点，但其在结肠癌（CC）中的意义尚不清楚。因此，本研究旨在确定RRP 15在CC中的表达和生物学功能。结果表明，与正常结肠标本相比，RRP 15在CC中强表达，这与患者的总生存期（OS）和无病生存期（DFS）较差相关。在所研究的9种CC细胞系中，RRP 15分别在HCT 15和HCT 116细胞中表现出最高和最低的表达。体外实验表明，RRP 15基因的敲低抑制了CC细胞的生长、集落形成能力和侵袭能力，而其过表达增强了上述致癌功能。此外，裸鼠皮下肿瘤显示RRP 15敲低抑制CC生长，而其过表达促进其生长。此外，RRP 15的敲低抑制了CC中的上皮-间充质转化（EMT），而RRP 15的过表达促进了CC中的EMT过程。总之，抑制RRP 15抑制肿瘤生长、侵袭和CC的EMT，并且可能被认为是治疗CC的有希望的治疗靶点。
Although ribosomal RNA processing 15 Homolog (RRP15) has been implicated in the occurrence of various cancers and is considered a potential target for cancer treatment, its significance in colon cancer (CC) is unclear. Thus, this present study aims to determine RRP15 expression and biological function in CC. The results demonstrated a strong expression of RRP15 in CC compared to normal colon specimens, which was correlated with poorer overall survival (OS) and disease-free survival (DFS) of the patients. Among the nine investigated CC cell lines, RRP15 demonstrated the highest and lowest expression in HCT15 and HCT116 cells, respectively. In vitro assays demonstrated that the knockdown of RRP15 inhibited the growth, colony-forming ability and invasive ability of the CC cells whereas its overexpression enhanced the above oncogenic function. Moreover, subcutaneous tumors in nude mice showed that RRP15 knockdown inhibited the CC growth while its overexpression enhanced their growth. Additionally, the knockdown of RRP15 inhibited the epithelial–mesenchymal transition (EMT), whereas overexpression of RRP15 promoted the EMT process in CC. Collectively, inhibition of RRP15 suppressed tumor growth, invasion and EMT of CC, and might be considered a promising therapeutic target for treating CC.
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影响因子：
2.6
作者：
Wu, Tao;Ren, Mei-Xia;Chen, Guo-ping;Jin, Zheng-ming;Wang, Gang
通讯作者：
Wang, Gang
circFNDC3B编码的新型蛋白质通过调节结肠癌中的Snail抑制肿瘤进展和EMT
影响因子：
37.3
作者：
Pan, Zihao;Cai, Jianye;Zhang, Lei
通讯作者：
Zhang, Lei
影响因子：
4.5
作者：
De Marchis, ML;Giorgi, A;Fatica, A
通讯作者：
Fatica, A
DOI：
10.1186/s13046-021-01828-7
发表时间：
2021-03-01
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
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作者：
Lee Y;Ko D;Yoon J;Lee Y;Kim S
通讯作者：
Kim S
影响因子：
11.2
作者：
通讯作者：
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