Rrp15 affects cell cycle, proliferation, and apoptosis in NIH3T3 cells.
Rrp15 affects cell cycle, proliferation, and apoptosis in NIH3T3 cells.
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DOI：
10.1002/2211-5463.12128
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发表时间：
2016-11
期刊：
影响因子：
2.6
通讯作者：
Wang, Gang
中科院分区：
文献类型：
--
作者：
Wu, Tao;Ren, Mei-Xia;Chen, Guo-ping;Jin, Zheng-ming;Wang, Gang
Riken 2810430M08（以下简称Rrp15）是新发现并报道的来自小鼠基因组的基因。在我们之前的工作中，我们发现该基因与T细胞的增殖和激活有关。Rrp15蛋白与芽殖酵母（Rrp15）高度同源，在核糖体RNA加工中起重要作用。我们探索了Rrp15在细胞凋亡、细胞增殖中的潜在功能，以及它与细胞核中RNA的关系。我们在NIH3T3细胞中构建了Rrp15基因的敲低，然后通过实时PCR、western blotting、流式细胞术和免疫荧光来确定Rrp15基因的功能。Rrp15基因敲低可抑制细胞增殖，诱导细胞凋亡。我们还发现Rrp15蛋白在细胞核中呈正态分布，并与细胞核中的RNA或前RNA结合。此外，Rrp15改变了20S蛋白酶体的活性。Rrp15在NIH3T3细胞中促进增殖和抑制凋亡，可能与细胞核中的RNA有关。
Riken 2810430M08 (hereinafter referred to as Rrp15) is a newly identified and reported gene from the mouse genome. In our previous work, we found that the gene had a relationship with the proliferation and activation of T cells. Rrp15 protein is highly homologous with RRP15 (budding yeast), which has an important role in ribosomal RNA processing. We explored the potential function of Rrp15 in apoptosis, cell proliferation, and its involvement with RNA in the nucleus. We constructed a knockdown of the Rrp15 gene in NIH3T3 cells and then performed real‐time PCR, western blotting, flow cytometry, and immunofluorescence to determine the function of the Rrp15 gene. Knockdown of the Rrp15 gene suppresses proliferation and induces apoptosis. We also found that the Rrp15 protein was normally distributed in the nucleus and bound to RNA or pre‐RNA in the nucleus. Additionally, Rrp15 altered the activity of the 20S proteasome. Rrp15 promotes proliferation and inhibits apoptosis in NIH3T3 cells and may have a relationship with RNA in the nucleus.
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影响因子：
4.5
作者：
De Marchis, ML;Giorgi, A;Fatica, A
通讯作者：
Fatica, A
影响因子：
4.8
作者：
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通讯作者：
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DOI：
10.1182/blood.v35.5.708.708
发表时间：
1970-01-01
期刊：
BLOOD-THE JOURNAL OF HEMATOLOGY
影响因子：
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作者：
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通讯作者：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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