Post-traumatic caspase-3 expression in the adjacent areas of growth plate injury site: a morphological study.
Post-traumatic caspase-3 expression in the adjacent areas of growth plate injury site: a morphological study.
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生长板损伤部位相邻区域的创伤后caspase-3表达：形态学研究。
DOI：
10.3390/ijms140815767
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发表时间：
2013-07-29
影响因子：
5.6
通讯作者：
Weinberg AM
中科院分区：
文献类型：
--
作者：
Musumeci G;Castrogiovanni P;Loreto C;Castorina S;Pichler K;Weinberg AM
骺板是位于骨干和骺之间的透明软骨板。本研究的目的是通过研究组织形态学、免疫组织化学、组织形态计量学和caspase-3和裂解的PARP-1的Western印迹分析，以及炎性细胞因子白细胞介素-6（IL-6）和肿瘤坏死因子α（TNF-α）的水平，以获得更多关于骨骺细胞更新的损伤后反应的信息。在我们的研究结果中，形态学分析表明，在实验骨中，新形成的骨小梁-由骨形成修复引起-侵入生长板并到达干骺端骨组织（骨桥），这可能导致某些生长停滞。我们通过ELISA证明，炎性细胞因子IL-6和TNF-α的表达水平增加。免疫组化、组织形态计量学和Western Blot分析显示，实验组骨组织中caspase-3和PARP-1的表达均显著高于对照组。总之，我们可以假设炎症过程对软骨细胞造成压力，这些软骨细胞将作为生物防御机制死亡，并且还将增加新软骨细胞的存活以维持细胞稳态。然而，确切的刺激导致增加的细胞凋亡率，观察损伤后，需要更多的研究，以了解软骨细胞凋亡的可能贡献的生长障碍。
The epiphyseal plate is a hyaline cartilage plate that sits between the diaphysis and the epiphysis. The objective of this study was to determine the impact of an injury in the growth plate chondrocytes through the study of histological morphology, immunohistochemistry, histomorphometry and Western Blot analyses of the caspase-3 and cleaved PARP-1, and levels of the inflammatory cytokines, Interleukin-6 (IL-6) and Tumor Necrosis Factor alpha (TNF-α), in order to acquire more information about post-injury reactions of physeal cell turnover. In our results, morphological analysis showed that in experimental bones, neo-formed bone trabeculae—resulting from bone formation repair—invaded the growth plate and reached the metaphyseal bone tissue (bone bridge), and this could result in some growth arrest. We demonstrated, by ELISA, increased expression levels of the inflammatory cytokines IL-6 and TNF-α. Immunohistochemistry, histomorphometry and Western Blot analyses of the caspase-3 and cleaved PARP-1 showed that the physeal apoptosis rate of the experimental bones was significantly higher than that of the control ones. In conclusion, we could assume that the inflammation process causes stress to chondrocytes that will die as a biological defense mechanism, and will also increase the survival of new chondrocytes for maintaining cell homeostasis. Nevertheless, the exact stimulus leading to the increased apoptosis rate, observed after injury, needs additional research to understand the possible contribution of chondrocyte apoptosis to growth disturbance.
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影响因子：
2.5
作者：
Leonardi, Rosalia;Rusu, Mugurel Constantin;Musumeci, Giuseppe
通讯作者：
Musumeci, Giuseppe
影响因子：
2.8
作者：
Aizawa, T;Kon, T;Gerstenfeld, LC
通讯作者：
Gerstenfeld, LC
影响因子：
2
作者：
Cardile, V.;Musumeci, G.;Loreto, C.
通讯作者：
Loreto, C.
影响因子：
2
作者：
Loreto, C.;Musumeci, G.;Leonardi, R.
通讯作者：
Leonardi, R.
影响因子：
2
作者：
Musumeci, Giuseppe;Loreto, Carla;Elisseeff, Jennifer
通讯作者：
Elisseeff, Jennifer