Preparation and properties of DNA-lipid complexes carrying pyrene and anthracene moieties
Preparation and properties of DNA-lipid complexes carrying pyrene and anthracene moieties
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携带芘和蒽部分的DNA-脂质复合物的制备和性质
DOI：
10.1016/j.eurpolymj.2010.12.010
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发表时间：
2011-03
影响因子：
6
通讯作者：
Masuda, Toshio
中科院分区：
文献类型：
--
作者：
Zhang, Tong;Qu, Jinqing;Ogata, Naoya;Masuda, Toshio
用阳离子型两亲性脂质PY和AnthDNA分别取代钠离子，制备了含芘和蒽的DNA-脂质复合物，其磷酸根与脂质的摩尔比分别为1：1.11和1：1.03。DNA-脂质（PY）和DNA-脂质（Anthh）复合物可溶于CHCl_3、CH_2Cl_2、甲醇和乙醇等有机溶剂，不溶于THF、甲苯和水溶液。CD光谱显示DNA-脂质（PY）和DNA-脂质（Anthh）复合物在甲醇中主要呈双螺旋结构，并且该螺旋结构对加热相当稳定。DNA-脂质（PY）复合物溶液的荧光量子产率为27.8%，高于相应脂质（PY）的荧光量子产率（16.8%），而DNA-脂质（Anthh）复合物溶液的荧光量子产率（45.4%）低于相应脂质（Anthh）的荧光量子产率（53.0%）。根据空气中的TGA，DNA-脂质（PY）和DNA-脂质（Anth）的重量损失的起始温度均为220°C。
Novel DNA–lipid complexes carrying pyrene and anthracene were prepared by substituting sodium counter cations with cationic amphiphilic lipids, namely lipid(PY) and lipid(Anth), in which the actual mole ratios of phosphate to lipid were 1:1.11 and 1:1.03, respectively. DNA–lipid(PY) and DNA–lipid(Anth) complexes were soluble in common organic solvents including CHCl3, CH2Cl2, methanol and ethanol, while insoluble in THF, toluene, and aqueous solutions. CD spectroscopy revealed that DNA–lipid(PY) and DNA–lipid(Anth) complexes took a predominantly double helical structure in methanol and that the helical structure was fairly stable against heating. The solution of DNA–lipid(PY) complex emitted fluorescence in 27.8% quantum yield, which were higher than that of the corresponding lipid(PY) (16.8%), while the fluorescence quantum yields of the solution of DNA–lipid(Anth) (45.4%) was lower than that of the lipid(Anth) (53.0%). The onset temperatures of weight loss of DNA–lipid(PY) and DNA–lipid(Anth) were both 220°C according to TGA in air.
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影响因子：
56.9
作者：
Radler, JO;Koltover, I;Safinya, CR
通讯作者：
Safinya, CR
影响因子：
4.9
作者：
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通讯作者：
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影响因子：
4.6
作者：
Qu, Jinqing;Qiu, Zhiming;Chen, Huanqin;Ogata, Naoya;Masuda, Toshio
通讯作者：
Masuda, Toshio
影响因子：
56.9
作者：
CARUTHERS, MH
通讯作者：
CARUTHERS, MH
影响因子：
5.5
作者：
M. Winnik;and Simon M. Bystryak;Zhaoqing Liu;J. Siddiqui
通讯作者：
M. Winnik;and Simon M. Bystryak;Zhaoqing Liu;J. Siddiqui