Early-stage bilayer tissue-engineered skin substitute formed by adult skin progenitor cells produces an improved skin structure in vivo.
Early-stage bilayer tissue-engineered skin substitute formed by adult skin progenitor cells produces an improved skin structure in vivo.
复制标题
由成人皮肤祖细胞形成的早期双层组织工程皮肤替代品可改善体内皮肤结构
DOI：
10.1186/s13287-020-01924-z
复制
发表时间：
2020-09-18
影响因子：
7.5
通讯作者：
Wu X
中科院分区：
文献类型：
--
作者：
Zhang Q;Wen J;Liu C;Ma C;Bai F;Leng X;Chen Z;Xie Z;Mi J;Wu X
BackgroundIn recent years, significant progress has been made in developing highly complex tissue-engineered skin substitutes (TESSs) for wound healing. However, the lack of skin appendages, such as hair follicles and sweat glands, and the time required, are two major limitations that hinder its broad application in the clinic. Therefore, it is necessary to develop a competent TESS in a short time to meet the needs for clinical applications.MethodsAdult scalp dermal progenitor cells and epidermal stem cells together with type I collagen as a scaffold material were used to reconstitute bilayer TESSs in vitro. TESSs at 4 different culture times (5, 9, 14, and 21 days) were collected and then grafted onto full-thickness wounds created in the dorsal skin of athymic nude/nude mice. The skin specimens formed from grafted TESSs were collected 4 and 8 weeks later and then evaluated for their structure, cell organization, differentiation status, vascularization, and formation of appendages by histological analysis, immunohistochemistry, and immunofluorescent staining.ResultsEarly-stage bilayer TESSs after transplantation had a better efficiency of grafting. A normal structure of stratified epidermis containing multiple differentiated layers of keratinocytes was formed in all grafts from both early-stage and late-stage TESSs, but higher levels of the proliferation marker Ki-67 and the epidermal progenitor marker p63 were found in the epidermis formed from early-stage TESSs. Interestingly, the transplantation of early-stage TESSs produced a thicker dermis that contained more vimentin- and CD31-positive cells, and importantly, hair follicle formation was only observed in the skin grafted from early-stage TESSs. Finally, early-stage TESSs expressed high levels of p63 but had low expression levels of genes involved in the activation of the apoptotic pathway compared to the late-stage TESSs in vitro.ConclusionsEarly-stage bilayer TESSs reconstituted from skin progenitor cells contained more competent cells with less activation of the apoptotic pathway and produced a better skin structure, including hair follicles associated with sebaceous glands, after transplantation, which should potentially provide better wound healing when applied in the clinic in the future.
登录
查看更多内容
影响因子：
--
作者：
Carlson, Mark W;Alt-Holland, Addy;Egles, Christophe;Garlick, Jonathan A
通讯作者：
Garlick, Jonathan A
DOI：
10.1073/pnas.1309970110
发表时间：
2013-12-03
影响因子：
11.1
作者：
Higgins, Claire A.;Chen, James C.;Christiano, Angela M.
通讯作者：
Christiano, Angela M.
DOI：
10.1097/bcr.0000000000000401
发表时间：
2017
期刊：
Journal of burn care & research : official publication of the American Burn Association
影响因子：
--
作者：
Boyce ST;Simpson PS;Rieman MT;Warner PM;Yakuboff KP;Bailey JK;Nelson JK;Fowler LA;Kagan RJ
通讯作者：
Kagan RJ
影响因子：
56.9
作者：
LANGER, R;VACANTI, JP
通讯作者：
VACANTI, JP
影响因子：
10.5
作者：
Koster, MI;Kim, S;Roop, DR
通讯作者：
Roop, DR