High-throughput screening of chemical exchange saturation transfer MR contrast agents.
High-throughput screening of chemical exchange saturation transfer MR contrast agents.
复制标题
DOI：
10.1002/cmmi.383
复制
发表时间：
2010-05
影响因子：
--
通讯作者：
McMahon, Michael T.
中科院分区：
文献类型：
--
作者：
Liu, Guanshu;Gilad, Assaf A.;Bulte, Jeff W. M.;van Zijl, Peter C. M.;McMahon, Michael T.
介绍了一种用于筛选化学交换饱和转移(CEST)试剂的高通量(HT)MRI新方法，该方法允许同时测试多个样品，最大限度地减少了对样品结构和主磁场(B0)垫片的关注。这种方法适用于抗磁性(DIACEST)、顺磁性(PARACEST)和脂质体(LIPOCEST)CEST试剂，它使用一套廉价的装有CEST试剂的玻璃或塑料毛细管，将CEST试剂放在一个廉价的塑料管支架中，而不需要在管之间放置液体，以减少磁化率影响。在此设置中，获取直接含水饱和度光谱的图像，以便为样本图像中的每个体积元素(体素)映射绝对水频率，然后获取饱和传递光谱图像以确定CEST性质。尽管由于空气/管道界面的原因，整个样品上的场非常不均匀，但直接饱和光谱的形状不受影响，允许通过使用地图上的绝对水频率来消除CEST数据中的磁化率漂移效应。因此，可以一次为多个CEST试剂提取诸如每个样品的平均CEST强度等定量信息。作为最初的应用，我们展示了16个多肽的CEST性质的快速筛选，但原则上，管的数量仅受可用信噪比、视野和成像的梯度强度的限制。
A new high throughput (HT) MRI method for screening Chemical Exchange Saturation Transfer (CEST) agents is demonstrated, allowing simultaneous testing of multiple samples with minimal attention to sample configuration and shimming of the main magnetic field (B0). This approach, which is applicable to diamagnetic (DIACEST), paramagnetic (PARACEST) and liposome (LIPOCEST) CEST agents, employs a set of inexpensive glass or plastic capillary tubes containing CEST agents put together in a cheap plastic tube holder, without need for liquid between the tubes to reduce magnetic susceptibility effects. In this setup, a image of direct water saturation spectra is acquired in order to map the absolute water frequency for each volume element (voxel) in the sample image, followed by an image of saturation transfer spectra to determine the CEST properties. Even though the field over the total sample is very inhomogeneous due to air/tube interfaces, the shape of the direct saturation spectra is not affected, allowing removal of susceptibility shift effects from the CEST data by using the absolute water frequencies from the map. As a result, quantitative information such as the mean CEST intensity for each sample can be extracted for multiple CEST agents at once. As an initial application, we demonstrate rapid screening of a library of 16 polypeptides for their CEST properties, but in principle the number of tubes is limited only by the available signal-noise-ratio, field of view and gradient strength for imaging.
登录
查看更多内容
影响因子：
3.3
作者：
McMahon, Michael T.;Gilad, Assaf A.;DeLiso, Marco A.;Berman, Stacey D. Cromer;Bulte, Jeff W. M.;van Zijl, Peter C. M.
通讯作者：
van Zijl, Peter C. M.
影响因子：
3.3
作者：
Liu, Guanshu;Li, Yuguo;Pagel, Mark D.
通讯作者：
Pagel, Mark D.
影响因子：
15
作者：
Langereis, Sander;Keupp, Jochen;Gruell, Holger
通讯作者：
Gruell, Holger
影响因子：
16.6
作者：
Chauvin, Thomas;Durand, Philippe;Toth, Eva
通讯作者：
Toth, Eva
影响因子：
--
作者：
Stancanello, J.;Terreno, E.;Aime, S.
通讯作者：
Aime, S.