Standardized two-step testing of antibody activity in COVID-19 convalescent plasma.
Standardized two-step testing of antibody activity in COVID-19 convalescent plasma.
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DOI：
10.1016/j.isci.2021.103602
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发表时间：
2022-01-21
期刊：
影响因子：
5.8
通讯作者：
Carnahan RH
中科院分区：
文献类型：
--
作者：
Gilchuk P;Thomsen I;Yoder S;Brady E;Chappell JD;Stevens LJ;Denison MR;Sutton RE;Chen RE;VanBlargan LA;Suryadevara N;Zost SJ;Schmitz J;Pulley JM;Diamond MS;Rhoads JP;Bernard GR;Self WH;Rice TW;Wheeler AP;Crowe JE Jr;Carnahan RH
COVID-19大流行揭示了对中和抗体反应的快速分析和抗体疗法的开发的迫切需要。目前美国食品和药物管理局批准的血清学试验不测量抗体介导的病毒中和，并且需要标准化的定量中和测定。我们报告了一个高通量的两步分析方法，用于确定中和恢复期血浆。在实时细胞分析测定中，使用表达SARS-CoV-2刺突蛋白的嵌合水泡性口炎病毒，对结合受体结合结构域的血清抗体进行筛选和向下选择，然后进行定量中和试验。这种方法能够实现用于鉴定中和SARS-CoV-2的血浆单位的预测筛选过程。为了校准来自恢复期血浆供体的血清中的抗体中和活性，我们引入了由两种人抗体组成的中和抗体标准试剂，其中和SARS-CoV毒株，包括关注的SARS-CoV-2变体。我们的研究结果为建立基于抗体的COVID-19干预措施的标准化评估提供了框架。一种高通量方法，可在COVID-19血浆SARS-CoV-2 IgG结合筛查中进行抗体活性测试，并结合定量中和测试。中和测试是分析COVID-19恢复期血浆的必要条件。两种广泛的单克隆抗体被确定为中和测试标准。免疫学;病毒学
The COVID-19 pandemic revealed an urgent need for rapid profiling of neutralizing antibody responses and development of antibody therapeutics. The current Food and Drug Administration-approved serological tests do not measure antibody-mediated viral neutralization, and there is a need for standardized quantitative neutralization assays. We report a high-throughput two-step profiling approach for identifying neutralizing convalescent plasma. Screening and downselection for serum antibody binding to the receptor-binding domain are followed by quantitative neutralization testing using a chimeric vesicular stomatitis virus expressing spike protein of SARS-CoV-2 in a real-time cell analysis assay. This approach enables a predictive screening process for identifying plasma units that neutralize SARS-CoV-2. To calibrate antibody neutralizing activity in serum from convalescent plasma donors, we introduce a neutralizing antibody standard reagent composed of two human antibodies that neutralize SARS-CoV strains, including SARS-CoV-2 variants of concern. Our results provide a framework for establishing a standardized assessment of antibody-based interventions against COVID-19. A high-throughput approach enabling antibody activity testing in COVID-19 plasma SARS-CoV-2 IgG binding screen coupled with quantitative neutralization testing Neutralization testing is necessary for profiling COVID-19 convalescent plasma Two broad monoclonal antibodies identified as a neutralization testing standard Immunology; Virology
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影响因子：
2.9
作者：
Gniadek TJ;Thiede JM;Matchett WE;Gress AR;Pape KA;Fiege JK;Jenkins MK;Menachery VD;Langlois RA;Bold TD
通讯作者：
Bold TD
影响因子：
9.4
作者：
Addetia A;Crawford KHD;Dingens A;Zhu H;Roychoudhury P;Huang ML;Jerome KR;Bloom JD;Greninger AL
通讯作者：
Greninger AL
影响因子：
30.3
作者：
Case, James Brett;Rothlauf, Paul W.;Whelan, Sean P. J.
通讯作者：
Whelan, Sean P. J.
影响因子：
17.1
作者：
Jones BE;Brown-Augsburger PL;Corbett KS;Westendorf K;Davies J;Cujec TP;Wiethoff CM;Blackbourne JL;Heinz BA;Foster D;Higgs RE;Balasubramaniam D;Wang L;Zhang Y;Yang ES;Bidshahri R;Kraft L;Hwang Y;Žentelis S;Jepson KR;Goya R;Smith MA;Collins DW;Hinshaw SJ;Tycho SA;Pellacani D;Xiang P;Muthuraman K;Sobhanifar S;Piper MH;Triana FJ;Hendle J;Pustilnik A;Adams AC;Berens SJ;Baric RS;Martinez DR;Cross RW;Geisbert TW;Borisevich V;Abiona O;Belli HM;de Vries M;Mohamed A;Dittmann M;Samanovic MI;Mulligan MJ;Goldsmith JA;Hsieh CL;Johnson NV;Wrapp D;McLellan JS;Barnhart BC;Graham BS;Mascola JR;Hansen CL;Falconer E
通讯作者：
Falconer E
REGN-COV2抗体预防和治疗恒河猕猴和仓鼠中的SARS-COV-2感染。
DOI：
10.1126/science.abe2402
发表时间：
2020-11-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Baum A;Ajithdoss D;Copin R;Zhou A;Lanza K;Negron N;Ni M;Wei Y;Mohammadi K;Musser B;Atwal GS;Oyejide A;Goez-Gazi Y;Dutton J;Clemmons E;Staples HM;Bartley C;Klaffke B;Alfson K;Gazi M;Gonzalez O;Dick E Jr;Carrion R Jr;Pessaint L;Porto M;Cook A;Brown R;Ali V;Greenhouse J;Taylor T;Andersen H;Lewis MG;Stahl N;Murphy AJ;Yancopoulos GD;Kyratsous CA
通讯作者：
Kyratsous CA