The α-subunit of the trimeric GTPase Go2 regulates axonal growth.
The α-subunit of the trimeric GTPase Go2 regulates axonal growth.
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DOI：
10.1111/jnc.12123
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发表时间：
2013-03
影响因子：
4.7
通讯作者：
Brunk I
中科院分区：
文献类型：
--
作者：
Baron J;Blex C;Rohrbeck A;Rachakonda SK;Birnbaumer L;Ahnert-Hilger G;Brunk I
Goα剪接变异体Go 1α和Go 2α是大脑中含量最丰富的G蛋白Go 1和Go 2的亚基。到目前为止，只描述了几个与go1α结合的相互作用伙伴，剪接变体的具体差异尚不清楚。用酵母双杂交筛选GO_2α为诱饵，用免疫共沉淀法确定Rap1GTP酶激活蛋白(Rap1GTP)和吉尔丁为GO_2α的相互作用伙伴。比较野生型和GO2型α−/−小鼠大脑的亚细胞部分，发现GRADIN或RAP1GAP的总体表达水平没有差异。然而，我们在Go2α缺失突变体的脑中发现了更多的活性RAP1GTP，这表明GO2GAP可能增加了RAP1GAP的活性，从而影响了RAP1的激活/失活周期。RAP1已被证明与轴突生长有关，并且考虑到RAP1GAP-GO2α的相互作用，我们发现GO2α的缺失影响了轴突的生长。与野生型小鼠相比，胚胎Go2α−/−小鼠培养的皮层和海马神经元轴突生长更长，形成更多的分支。综上所述，我们提供了Go2α调控轴突生长和分支的证据。
The Goα splice variants Go1α and Go2α are subunits of the most abundant G-proteins in brain, Go1 and Go2. Only a few interacting partners binding to Go1α have been described so far and splice variant specific differences are not known. Using a yeast two-hybrid screen with constitutively active Go2α as bait, we identified Rap1GTPase activating protein (Rap1GAP) and Girdin as interacting partners of Go2α, which was confirmed by co-immunoprecipitation. Comparison of subcellular fractions from brains of wild type and Go2α−/− mice revealed no differences in the overall expression level of Girdin or Rap1GAP. However, we found higher amounts of active Rap1-GTP in brains of Go2α deficient mutants, indicating that Go2α may increase Rap1GAP activity, thereby effecting the Rap1 activation/deactivation cycle. Rap1 has been shown to be involved in neurite outgrowth and given a Rap1GAP-Go2α interaction, we found that the loss of Go2α affected axonal outgrowth. Axons of cultured cortical and hippocampal neurons prepared from embryonic Go2α−/− mice grew longer and developed more branches than those from wild type mice. Taken together we provide evidence that Go2α regulates axonal outgrowth and branching.
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影响因子：
2.1
作者：
He, John Cijiang;Neves, Susana R.;Iyengar, Ravi
通讯作者：
Iyengar, Ravi
影响因子：
3.5
作者：
Kerov, VS;Natochin, M;Artemyev, NO
通讯作者：
Artemyev, NO
影响因子：
5.3
作者：
Dhingra, A;Jiang, MS;Vardi, N
通讯作者：
Vardi, N
影响因子：
4.8
作者：
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通讯作者：
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影响因子：
11.4
作者：
Ahnert-Hilger, G;Nürnberg, B;Jahn, R
通讯作者：
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