In vitro studies on the effect of particle size on macrophage responses to nanodiamond wear debris.
In vitro studies on the effect of particle size on macrophage responses to nanodiamond wear debris.
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DOI：
10.1016/j.actbio.2012.01.033
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发表时间：
2012-05
影响因子：
9.7
通讯作者：
Vohra, Yogesh K.
中科院分区：
文献类型：
--
作者：
Thomas, Vinoy;Halloran, Brian A.;Ambalavanan, Namasivayam;Catledge, Shane A.;Vohra, Yogesh K.
纳米结构金刚石涂层改善了全髋关节置换术金属部件的光滑度和磨损特性，并延长了这些植入物的寿命，但需要确定纳米金刚石磨屑对巨噬细胞的影响，以估计磨屑的长期炎症效应。目的是研究合成纳米金刚石颗粒的尺寸对巨噬细胞增殖（BrdU掺入）、凋亡（Annexin-V流式细胞术）、代谢活性（WST-1测定）和炎性细胞因子产生（qPCR）的影响。将RAW 264.7巨噬细胞暴露于不同尺寸（6、60、100、250和500 nm）和浓度（0、10、50、100和200 μg ml-1）的合成纳米金刚石。我们观察到，在较低浓度（50 μg ml−1）下，6-100 nm的纳米颗粒尺寸降低了细胞增殖，但没有降低代谢活性，并且在200 μg ml−1的纳米金刚石浓度下，细胞增殖和代谢活性都显著降低。流式细胞术表明，由于坏死，无论颗粒大小，细胞活力显着降低。与仅含血清的对照组或氧化钛（108 nm）纳米颗粒相比，纳米金刚石暴露显著降低了肿瘤坏死因子-α、白细胞介素-1 β、趋化因子Ccl 2和血小板衍生生长因子的基因表达，对趋化因子Cxcl 2和血管内皮生长因子的影响不同。总的来说，我们的研究证明了体外巨噬细胞对纳米金刚石颗粒的反应具有尺寸和浓度依赖性，纳米金刚石颗粒可能是金刚石涂层骨科关节植入物的磨损碎屑。
Nanostructured diamond coatings improve the smoothness and wear characteristics of the metallic component of total hip replacements and increase the longevity of these implants, but the effect of nanodiamond wear debris on macrophages needs to be determined to estimate the long-term inflammatory effects of wear debris. The objective was to investigate the effect of the size of synthetic nanodiamond particles on macrophage proliferation (BrdU incorporation), apoptosis (Annexin-V flow cytometry), metabolic activity (WST-1 assay) and inflammatory cytokine production (qPCR). RAW 264.7 macrophages were exposed to varying sizes (6, 60, 100, 250 and 500 nm) and concentrations (0, 10, 50, 100 and 200 μg ml−1) of synthetic nanodiamonds. We observed that cell proliferation but not metabolic activity was decreased with nanoparticle sizes of 6–100 nm at lower concentrations (50 μg ml−1), and both cell proliferation and metabolic activity were significantly reduced with nanodiamond concentrations of 200 μg ml−1. Flow cytometry indicated a significant reduction in cell viability due to necrosis irrespective of particle size. Nanodiamond exposure significantly reduced gene expression of tumor necrosis factor-α, interleukin-1β, chemokine Ccl2 and platelet-derived growth factor compared to serum-only controls or titanium oxide (anatase 8 nm) nanoparticles, with variable effects on chemokine Cxcl2 and vascular endothelial growth factor. In general, our study demonstrates a size and concentration dependence of macrophage responses in vitro to nanodiamond particles as possible wear debris from diamond-coated orthopedic joint implants.
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影响因子：
2.8
作者：
Algan, SM;Purdon, M;Horowitz, SM
通讯作者：
Horowitz, SM
影响因子：
1.5
作者：
Catledge, SA;Thomas, V;Vohra, YK
通讯作者：
Vohra, YK
影响因子：
13.3
作者：
Alhaddad, Anna;Adam, Marie-Pierre;Bertrand, Jean-Remi
通讯作者：
Bertrand, Jean-Remi
DOI：
10.1002/jbm.b.30926
发表时间：
2008-04-01
影响因子：
3.4
作者：
Hill, Michael R.;Catledge, Shane A.;Eberhardt, Alan W.
通讯作者：
Eberhardt, Alan W.
DOI：
10.2106/00004623-198365050-00001
发表时间：
1983-01-01
影响因子：
5.3
作者：
GOLDRING, SR;SCHILLER, AL;HARRIS, WH
通讯作者：
HARRIS, WH