Charging the quantum capacitance of graphene with a single biological ion channel.
Charging the quantum capacitance of graphene with a single biological ion channel.
复制标题
用单个生物离子通道充电石墨烯的量子电容。
DOI：
10.1021/nn501376z
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发表时间：
2014-05-27
期刊：
影响因子：
17.1
通讯作者：
Burke, Peter J.
中科院分区：
文献类型：
--
作者：
Wang, Yung Yu;Pham, Ted D.;Zand, Katayoun;Li, Jinfeng;Burke, Peter J.
细胞和细胞器膜（脂质双层）与纳米电子学的相互作用可以使新技术以新的定性方式感知和测量电生理。迄今为止，各种传感装置已被证明可以通过宏观数量的离子通道来测量膜电流。然而，基于纳米电子的单离子通道电流传感一直是一个挑战。在这里，我们报道了基于石墨烯的场效应晶体管与支持的脂质双层相结合，首次作为测量单个离子通道活性的平台。我们发现，支撑的脂质双层均匀地覆盖在单层石墨烯表面，作为一种仿生屏障，将石墨烯与电解质环境（电和化学）隔离开来。在引入诸如alamethicin和gramicidin A等成孔膜蛋白后，可以观察到电流脉冲通过从石墨烯到电解质的脂质双分子层，从而为石墨烯的量子电容充电。这种方法将纳米技术与电生理学相结合，展示了测量离子通道电流的定性新方法。
The interaction of cell and organelle membranes (lipid bilayers) with nanoelectronics can enable new technologies to sense and measure electrophysiology in qualitatively new ways. To date, a variety of sensing devices have been demonstrated to measure membrane currents through macroscopic numbers of ion channels. However, nanoelectronic based sensing of single ion channel currents has been a challenge. Here, we report graphene-based field-effect transistors combined with supported lipid bilayers as a platform for measuring, for the first time, individual ion channel activity. We show that the supported lipid bilayers uniformly coat the single layer graphene surface, acting as a biomimetic barrier that insulates (both electrically and chemically) the graphene from the electrolyte environment. Upon introduction of pore-forming membrane proteins such as alamethicin and gramicidin A, current pulses are observed through the lipid bilayers from the graphene to the electrolyte, which charge the quantum capacitance of the graphene. This approach combines nanotechnology with electrophysiology to demonstrate qualitatively new ways of measuring ion channel currents.
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