Response differences of HepG2 and Primary Mouse Hepatocytes to morphological changes in electrospun PCL scaffolds.
Response differences of HepG2 and Primary Mouse Hepatocytes to morphological changes in electrospun PCL scaffolds.
复制标题
HepG2和原代小鼠肝细胞对电纺丝PCL支架形态变化的反应差异。
DOI：
10.1038/s41598-021-81761-z
复制
发表时间：
2021-02-04
影响因子：
4.6
通讯作者：
Callanan A
中科院分区：
文献类型：
--
作者：
Bate TSR;Gadd VL;Forbes SJ;Callanan A
肝脏疾病病例在地球仪上迅速扩大，而治疗终末期疾病的唯一有效方法是移植。移植手术费用昂贵，目前供体肝脏的供应不能满足需求。为解决这些紧迫问题而开发的潜在药物治疗和再生疗法需要有效的体外培养平台。静电纺丝支架提供了生物模拟结构，在其上培养细胞以调节体外功能。本研究旨在阐明静电纺丝PCL形态对永生化肝细胞系和小鼠原代肝细胞培养的影响。每种细胞类型在具有随机、对齐和高度多孔的低温纺丝配置的4-5 μm大纤维和1-2 μm小纤维上培养。分析细胞的粘附、增殖、形态和功能蛋白及基因表达。结果表明，纤维形态对细胞形态和功能具有可测量的影响，并改变了关键功能标志物，如CYP 1A 2表达。
Liver disease cases are rapidly expanding across the globe and the only effective cure for end-stage disease is a transplant. Transplant procedures are costly and current supply of donor livers does not satisfy demand. Potential drug treatments and regenerative therapies that are being developed to tackle these pressing issues require effective in-vitro culture platforms. Electrospun scaffolds provide bio-mimetic structures upon which cells are cultured to regulate function in-vitro. This study aims to shed light on the effects of electrospun PCL morphology on the culture of an immortalised hepatic cell line and mouse primary hepatocytes. Each cell type was cultured on large 4–5 µm fibres and small 1–2 µm fibres with random, aligned and highly porous cryogenically spun configurations. Cell attachment, proliferation, morphology and functional protein and gene expression was analysed. Results show that fibre morphology has a measurable influence on cellular morphology and function, with the alteration of key functional markers such as CYP1A2 expression.
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DOI：
10.1016/j.msec.2006.05.019
发表时间：
2007-04-01
期刊：
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
影响因子：
--
作者：
Lannutti, J.;Reneker, D.;Farson, D. F.
通讯作者：
Farson, D. F.
影响因子：
4.3
作者：
Cozzolino AM;Noce V;Battistelli C;Marchetti A;Grassi G;Cicchini C;Tripodi M;Amicone L
通讯作者：
Amicone L
影响因子：
5.8
作者：
Goasduff, T;Dreano, Y;Berthou, F
通讯作者：
Berthou, F
DOI：
10.1073/pnas.86.20.7696
发表时间：
1989-10-01
影响因子：
11.1
作者：
BUTLER, MA;IWASAKI, M;KADLUBAR, FF
通讯作者：
KADLUBAR, FF
影响因子：
4.1
作者：
Accardi, Mario Alberto;McCullen, Seth D.;Dini, Daniele
通讯作者：
Dini, Daniele