Plasma proteoforms of apolipoproteins C-I and C-II are associated with plasma lipids in the Multi-Ethnic Study of Atherosclerosis.
Plasma proteoforms of apolipoproteins C-I and C-II are associated with plasma lipids in the Multi-Ethnic Study of Atherosclerosis.
复制标题
在动脉粥样硬化的多种族研究中，载脂蛋白 C-I 和 C-II 的血浆蛋白形式与血浆脂质相关。
DOI：
10.1016/j.jlr.2022.100263
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发表时间：
2022-09
影响因子：
6.5
通讯作者：
Reaven, Peter D.
中科院分区：
文献类型：
--
作者：
Koska, Juraj;Furtado, Jeremy;Hu, Yueming;Sinari, Shripad;Budoff, Matthew J.;Billheimer, Dean;Nedelkov, Dobrin;McClelland, Robyn L.;Reaven, Peter D.
关键词：
载脂蛋白 (apo) C-I 和 C-II 是甘油三酯和 HDL 代谢的关键调节因子。两者均以全尺寸天然蛋白和截短蛋白（apoC-I'；apoC-II'）翻译后蛋白形式存在。然而，这些蛋白质形式在脂质代谢中的决定因素和作用尚不清楚。在这里，我们通过质谱免疫测定法测量了来自动脉粥样硬化多种族研究的基线和 10 年随访血浆样本中的 apoC-I 和 apoC-II 蛋白质形式。我们发现，非裔美国人 (AA)、华裔美国人 (CA) 和西班牙裔美国人的基线总 apoC-I（平均值 = 9.2 mg/dl）较低（与白人相比，低 1.8、1.0、1.0 mg/dl），女性较高（低 1.2 mg/dl），并且与血浆甘油三酯和 HDL 呈正相关。此外，我们观察到 CA 中截短的 apoC-I 与天然 apoC-I 的比率 (apoC-I'/C-I) 较低，与甘油三酯呈负相关，与 HDL 呈正相关。我们确定，AA 人群中的总 apoC-II (8.8 mg/dl) 较低（低 0.8 mg/dl），而 CA 和西班牙裔人群则较高（分别高 0.5 和 0.4 mg/dl），与甘油三酯呈正相关，与 HDL 呈负相关。此外，apoC-II'/C-II 在 AA 和女性中较高，与甘油三酯呈负相关，与 HDL 呈正相关。我们发现甘油三酯的变化与总apoC-I和apoC-II的变化呈正相关，与apoC-I'/C-I和apoC-II'/C-II的变化呈负相关，而HDL的变化与总apoC-I和apoC-II'/C-II的变化呈正相关，与总apoC-II的变化呈负相关。这项研究记录了 apoC-I 和 apoC-II 血浆水平的种族/民族差异，并强调载脂蛋白翻译后修饰作为血浆脂质的潜在调节剂。
Apolipoproteins (apo) C-I and C-II are key regulators of triglyceride and HDL metabolism. Both exist as full-size native and truncated (apoC-I'; apoC-II') posttranslational proteoforms. However, the determinants and the role of these proteoforms in lipid metabolism are unknown. Here, we measured apoC-I and apoC-II proteoforms by mass spectrometry immunoassay in baseline and 10-year follow-up plasma samples from the Multi-Ethnic Study of Atherosclerosis. We found that baseline total apoC-I (mean = 9.2 mg/dl) was lower in African Americans (AA), Chinese Americans (CA), and Hispanics (by 1.8; 1.0; 1.0 mg/dl vs. whites), higher in women (by 1.2 mg/dl), and positively associated with plasma triglycerides and HDL. Furthermore, we observed that the truncated-to-native apoC-I ratio (apoC-I'/C-I) was lower in CA, negatively associated with triglycerides, and positively associated with HDL. We determined that total apoC-II (8.8 mg/dl) was lower in AA (by 0.8 mg/dl) and higher in CA and Hispanics (by 0.5 and 0.4 mg/dl), positively associated with triglycerides, and negatively associated with HDL. In addition, apoC-II'/C-II was higher in AA and women, negatively associated with triglycerides, and positively associated with HDL. We showed that the change in triglycerides was positively associated with changes in total apoC-I and apoC-II and negatively associated with changes in apoC-I'/C-I and apoC-II'/C-II, whereas the change in HDL was positively associated with changes in total apoC-I and apoC-II'/C-II and negatively associated with change in total apoC-II. This study documents racial/ethnic variation in apoC-I and apoC-II plasma levels and highlights apolipoprotein posttranslational modification as a potential regulator of plasma lipids.
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DOI：
10.1016/0006-2944(79)90063-2
发表时间：
1979-01-01
期刊：
BIOCHEMICAL MEDICINE
影响因子：
--
作者：
HAVEL, RJ;KOTITE, L;KANE, JP
通讯作者：
KANE, JP
DOI：
10.1161/atvbaha.109.197830
发表时间：
2010-02
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Mendivil CO;Zheng C;Furtado J;Lel J;Sacks FM
通讯作者：
Sacks FM
影响因子：
4.8
作者：
Larsson, Mikael;Vorrsjo, Evelina;Olivecrona, Gunilla
通讯作者：
Olivecrona, Gunilla
影响因子：
37.8
作者：
Bild, DE;Detrano, R;Saad, MF
通讯作者：
Saad, MF
影响因子：
5
作者：
EKMAN, R;NILSSONEHLE, P
通讯作者：
NILSSONEHLE, P