Basolateral Amygdala Regulates EEG Theta-activity During Rapid Eye Movement Sleep.
Basolateral Amygdala Regulates EEG Theta-activity During Rapid Eye Movement Sleep.
复制标题
基底外侧杏仁核调节快速眼动睡眠期间的EEG Theta活动。
DOI：
10.1016/j.neuroscience.2021.06.018
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发表时间：
2021-08-01
期刊：
影响因子：
3.3
通讯作者：
Sanford LD
中科院分区：
文献类型：
--
作者：
Machida M;Sweeten BLW;Adkins AM;Wellman LL;Sanford LD
Pharmacological and optogenetic studies have demonstrated that the basolateral amygdala (BLA) plays a pivotal role in regulating fear-conditioned changes in sleep, in particular, rapid eye movement sleep (REM). However, the linkage between BLA and REM regulation has been minimally examined. In this study, we optogenetically activated or inhibited BLA selectively during spontaneous REM, and determined the effects on REM amounts and on hippocampus regulated EEG-theta (θ) activity. Excitatory (CaMKIIα-hChR2 (E123A)-eYFP-WPRE) or inhibitory (CaMKIIα-eNpHR3.0-eYFP-WPRE) optogenetic constructs were stereotaxically delivered targeting glutamatergic cells in BLA (BLAGlu) of mice. Viral constructs without opsin (CaMKIIα-eYFP-WPRE) were used as controls. All mice were implanted with telemetry transmitters for monitoring electroencephalography (EEG), activity, and body temperature, and with optic cannulas for light delivery to the BLA. BLAGlu were optogenetically activated by blue light (473 nm), or inhibited by green light (532 nm), in 10 sec epochs during REM, or non-REM (NREM), in undisturbed mice. Sleep amounts and EEG activity were analyzed. Projections from BLAGlu to neurons in hippocampus were immunohistochemically (IHC) examined. Brief optic activation of BLAGlu during REM immediately reduced EEG theta activity (5 – 8 Hz, REM-θ), without affecting overall amount and propensity of sleep, while optic inhibition increased REM-θ. Stimulation during NREM had no effect on EEG spectra or sleep. IHC results showed that glutamatergic and GABAergic cells in CA3 of the hippocampus received inputs from BLAGlu projection neurons. Activation of BLAGlu reduced, and inhibition increased, REM-θ without otherwise altering sleep. Optogenetic stimulation of BLAGlu may be useful for systematically manipulating sleep-related amygdalo-hippocampal interactions.
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影响因子：
56.9
作者：
LARSON, J;LYNCH, G
通讯作者：
LYNCH, G
影响因子：
8.2
作者：
KLEMM, WR;NAUGLE, NW
通讯作者：
NAUGLE, NW
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Kossut, Malgorzata