Variability of nm23-H1/NDPK-A expression in human lymphomas and its relation to tumour aggressiveness.
Variability of nm23-H1/NDPK-A expression in human lymphomas and its relation to tumour aggressiveness.
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人类淋巴瘤中 nm23-H1/NDPK-A 表达的变异性及其与肿瘤侵袭性的关系。
DOI：
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发表时间：
1996
影响因子：
8.8
通讯作者：
Heinrich Kovar
中科院分区：
文献类型：
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作者：
D. Aryee;Ingrid Simonitsch;Isabella Mosberger;K. Kos;Georg Mann;E. Schlögl;U. Pötschger;Helmut Gadner;Thaddäus Radaszkiewicz;Heinrich Kovar
nm 23-H1基因是一个假定的转移抑制基因，编码一个17 kDa的具有核苷二磷酸激酶活性的蛋白。nm 23-H1/NDPK-A的表达与某些人类肿瘤和实验动物细胞的转移潜能呈负相关。目前还没有nm 23在人类恶性淋巴瘤中表达的研究。在这项研究中，我们研究了nm 23-H1的表达北方和免疫组化分析在106原发性淋巴瘤患者霍奇金病（HD）（n = 15），高级别非霍奇金淋巴瘤（NHL）从不同的血统（n = 71）和低级别NHL（n = 20）。nm 23-H1/NDPK-A表达水平的亚型间和亚型内差异在所有疾病亚型中均得到证实。除了这种异质性之外，观察到高度恶性样品表达比低级别淋巴瘤更高的nm 23-H1/NDPK-A水平的总体趋势。成人和儿童HD和高级别NHL样本均表现出比仅在成人中发现的低级别NHL显著更高的NDPK-A表达。高nm 23-H1/NDPK-A水平的淋巴瘤样本并不总是反映肿瘤细胞的增殖活性，监测Ki-67抗原染色。采用逆转录-聚合酶链反应（RT-PCR）和单链构象多态性（SSCP）分析方法对50例标本进行nm 23-H1基因编码序列突变的检测。通过该筛选未发现突变。我们的研究结果表明nm 23-H1表达在淋巴瘤的疾病侵袭性中的作用。
The nm23-H1 gene is a putative metastasis-suppressor gene encoding a 17 kDa protein with nucleoside diphosphate kinase activity. Expression of nm23-H1/NDPK-A correlates inversely with the metastasising potential of some human tumours and experimental animal cells. No nm23 expression studies exist for human malignant lymphomas so far. In this study, we examined nm23-H1 expression by Northern and immunohistochemical analysis in 106 primary lymphoma samples from patients with Hodgkin's disease (HD) (n = 15), high-grade non-Hodgkin's lymphoma (NHL) from different lineages (n = 71) and low-grade NHL (n = 20). Both inter- and intra-subtype variations in nm23-H1/NDPK-A expression levels were demonstrated by all disease subtypes. Besides this heterogeneity, a general trend towards highly malignant samples expressing higher nm23-H1/NDPK-A, levels than the low-grade lymphomas was observed. Both adult and childhood HD and high-grade NHL samples exhibited significantly higher NDPK-A expression than the low-grade NHL found only in adults. High nm23-H1/NDPK-A levels in lymphoma samples did not always reflect proliferative activity of tumour cells as monitored by Ki-67 antigen staining. Fifty samples were further investigated for possible mutations in the nm23-H1 coding sequence by means of reverse transcriptase-polymerase chain reaction (RT-PCR) and single-strand conformation polymorphism (SSCP) analysis. No mutation was found by this screening. Our results suggest a role for nm23-H1 expression in the disease aggressiveness of lymphomas.
影响因子：
15.9
作者：
KEIM, D;HAILAT, N;HANASH, SM
通讯作者：
HANASH, SM
DOI：
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发表时间：
2006
期刊：
--
影响因子：
--
作者：
J. Stahl;A. Leone;A. Rosengard;L. Porter;C. Richter King;Patricia S. Steeg
通讯作者：
J. Stahl;A. Leone;A. Rosengard;L. Porter;C. Richter King;Patricia S. Steeg
影响因子：
10.3
作者：
STEEG, PS;BEVILACQUA, G;SOBEL, ME
通讯作者：
SOBEL, ME