Innate Immune Responses of Galleria mellonella to Mycobacterium bovis BCG Challenge Identified Using Proteomic and Molecular Approaches.
Innate Immune Responses of Galleria mellonella to Mycobacterium bovis BCG Challenge Identified Using Proteomic and Molecular Approaches.
复制标题
DOI：
10.3389/fcimb.2021.619981
复制
发表时间：
2021
影响因子：
5.7
通讯作者：
Newton SM
中科院分区：
文献类型：
--
作者：
Asai M;Sheehan G;Li Y;Robertson BD;Kavanagh K;Langford PR;Newton SM
昆虫大蜡螟幼虫最近被建立为结核分支杆菌复合体（MTBC）的非哺乳动物感染模型。为了进一步了解该模型的潜力，我们应用蛋白质组学（无标记定量）和转录组学（基因表达）方法来研究G.在168小时的时间过程中，用牛分枝杆菌BCG lux感染大蜡蚧。对感染幼虫血淋巴的蛋白质组学分析显示，蛋白质组在所有时间点（4、48、168小时）均发生明显变化。逆转录酶定量PCR证实诱导5个基因（gloverin，天蚕抗菌肽，IMPI，hemolin，和Hdd 11），其编码的蛋白质被发现是差异丰富的蛋白质组学分析。然而，基因表达和蛋白质丰度之间的趋势在很大程度上不一致（20%）。总体而言，数据与先前的表型观察结果一致，如分枝杆菌的血细胞内化（血红素/β-肌动蛋白）、肉芽肿样结构的形成（Hdd 11）和黑化（酚氧化酶激活酶3和丝氨酸蛋白酶抑制剂）。此外，G.还鉴定了结核病感染的大梅隆菌、小鼠、斑马鱼和体外细胞系模型的吞噬作用机制（β-肌动蛋白）。抗菌肽（Cecropins）与人表达的高效肽（h-CAP-18）具有相同的α-螺旋基序，在G.大蜡螟对感染的反应，为新型抗分枝杆菌药物提供了潜在的起点。我们相信，这些新的见解先天免疫反应进一步有助于验证这种成本效益和道德上可接受的昆虫模型，研究成员的MTBC。
The larvae of the insect Galleria mellonella, have recently been established as a non-mammalian infection model for the Mycobacterium tuberculosis complex (MTBC). To gain further insight into the potential of this model, we applied proteomic (label-free quantification) and transcriptomic (gene expression) approaches to characterise the innate immune response of G. mellonella to infection with Mycobacterium bovis BCG lux over a 168 h time course. Proteomic analysis of the haemolymph from infected larvae revealed distinct changes in the proteome at all time points (4, 48, 168 h). Reverse transcriptase quantitative PCR confirmed induction of five genes (gloverin, cecropin, IMPI, hemolin, and Hdd11), which encoded proteins found to be differentially abundant from the proteomic analysis. However, the trend between gene expression and protein abundance were largely inconsistent (20%). Overall, the data are in agreement with previous phenotypic observations such as haemocyte internalization of mycobacterial bacilli (hemolin/β-actin), formation of granuloma-like structures (Hdd11), and melanization (phenoloxidase activating enzyme 3 and serpins). Furthermore, similarities in immune expression in G. mellonella, mouse, zebrafish and in vitro cell-line models of tuberculosis infection were also identified for the mechanism of phagocytosis (β-actin). Cecropins (antimicrobial peptides), which share the same α-helical motif as a highly potent peptide expressed in humans (h-CAP-18), were induced in G. mellonella in response to infection, giving insight into a potential starting point for novel antimycobacterial agents. We believe that these novel insights into the innate immune response further contribute to the validation of this cost-effective and ethically acceptable insect model to study members of the MTBC.
登录
查看更多内容
DOI：
10.3791/4196
发表时间：
2012-11-18
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Avdesh A;Chen M;Martin-Iverson MT;Mondal A;Ong D;Rainey-Smith S;Taddei K;Lardelli M;Groth DM;Verdile G;Martins RN
通讯作者：
Martins RN
影响因子：
3.7
作者：
Alghoribi MF;Gibreel TM;Dodgson AR;Beatson SA;Upton M
通讯作者：
Upton M
影响因子：
3.4
作者：
Correa, Wilmar;Manrique-Moreno, Marcela;Brandenburg, Klaus
通讯作者：
Brandenburg, Klaus
影响因子：
4.4
作者：
Gandhe, Archana S.;John, Serene H.;Nagaraju, Javaregowda
通讯作者：
Nagaraju, Javaregowda
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y