Regular care and maintenance of a zebrafish (Danio rerio) laboratory: an introduction.
Regular care and maintenance of a zebrafish (Danio rerio) laboratory: an introduction.
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DOI：
10.3791/4196
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发表时间：
2012-11-18
期刊：
影响因子：
--
通讯作者：
Martins RN
中科院分区：
文献类型：
--
作者：
Avdesh A;Chen M;Martin-Iverson MT;Mondal A;Ong D;Rainey-Smith S;Taddei K;Lardelli M;Groth DM;Verdile G;Martins RN
该方案描述了斑马鱼实验室的定期护理和维护。现在，斑马鱼在遗传学、药理学和行为研究中越来越受欢迎。作为一种脊椎动物，斑马鱼与人类具有相当大的遗传序列相似性，并被用作各种人类疾病的动物模型。与其他常见的脊椎动物模型相比，斑马鱼的优势包括繁殖力高，维护成本低，胚胎透明，发育迅速。由于对斑马鱼研究的兴趣，建立和维护生产性斑马鱼养殖设施的需求也在增加。虽然文献可用于维护斑马鱼实验室，但缺乏简明的视频协议。该视频说明了斑马鱼幼鱼的定期圈养、喂养、繁殖和饲养的协议。这一过程将有助于研究人员了解斑马鱼饲养的自然行为和最佳条件，从而解决源于鱼类饲养条件的实验问题。该方案将对计划建立斑马鱼实验室的研究人员以及打算使用斑马鱼作为动物模型的研究生提供巨大的帮助。
This protocol describes regular care and maintenance of a zebrafish laboratory. Zebrafish are now gaining popularity in genetics, pharmacological and behavioural research. As a vertebrate, zebrafish share considerable genetic sequence similarity with humans and are being used as an animal model for various human disease conditions. The advantages of zebrafish in comparison to other common vertebrate models include high fecundity, low maintenance cost, transparent embryos, and rapid development. Due to the spur of interest in zebrafish research, the need to establish and maintain a productive zebrafish housing facility is also increasing. Although literature is available for the maintenance of a zebrafish laboratory, a concise video protocol is lacking. This video illustrates the protocol for regular housing, feeding, breeding and raising of zebrafish larvae. This process will help researchers to understand the natural behaviour and optimal conditions of zebrafish husbandry and hence troubleshoot experimental issues that originate from the fish husbandry conditions. This protocol will be of immense help to researchers planning to establish a zebrafish laboratory, and also to graduate students who are intending to use zebrafish as an animal model.
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影响因子：
1.3
作者：
Colwill, RM;Raymond, MP;Escudero, H
通讯作者：
Escudero, H
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF
DOI：
10.1073/pnas.1107156108
发表时间：
2011-09-13
影响因子：
11.1
作者：
Wolman, Marc A.;Jain, Roshan A.;Granato, Michael
通讯作者：
Granato, Michael
影响因子：
1.6
作者：
Delaney, M;Follet, C;Gerlach, G
通讯作者：
Gerlach, G
影响因子：
3.4
作者：
Eddins, Donnie;Petro, Ann;Levin, Edward D.
通讯作者：
Levin, Edward D.