A study of Docetaxel-induced effects in MCF-7 cells by means of Raman microspectroscopy.
A study of Docetaxel-induced effects in MCF-7 cells by means of Raman microspectroscopy.
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DOI：
10.1007/s00216-012-5887-9
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发表时间：
2012-05
影响因子：
4.3
通讯作者：
Popp, Juergen
中科院分区：
文献类型：
--
作者：
Hartmann, Katharina;Becker-Putsche, Melanie;Bocklitz, Thomas;Pachmann, Katharina;Niendorf, Axel;Roesch, Petra;Popp, Juergen
化疗的成功率很低，约为25%，因此，为个体患者选择最有效的细胞抑制药物并监测正在进行的化疗的效率是实现个性化治疗的重要步骤。因此，能够区分治疗和未治疗的癌细胞的客观方法将是至关重要的。在这项研究中，我们提供了分子的见解多西他赛诱导的MCF-7细胞，作为腺癌的模型系统，通过拉曼显微光谱结合强大的化学计量学方法。拉曼数据的分析分为两个步骤。在第一部分中，细胞器的形态，例如细胞核已经通过用k-均值聚类分析和人工神经网络分析拉曼光谱可视化，并与组织病理学金标准方法苏木精和伊红染色进行比较。该比较表明，拉曼显微镜能够显示细胞形态;然而，这与无标记的苏木精和伊红染色相反，因此可以潜在地应用于体内。因为多西他赛是在细胞核内作用的药物，所以在下一步中进一步研究源自细胞核区域的拉曼光谱。因此，我们能够区分处理的未处理的MCF-7细胞，并通过利用线性判别分析模型来量化细胞-药物反应。拉曼显微光谱结合强大的化学计量学方法（如人工神经网络）表明形态（核碎裂）和光谱变化多西他赛处理的乳腺癌细胞（MCF-7）相比，未经处理的细胞样品这篇文章的在线版本（doi：10.1007/s 00216 -012-5887-9）包含补充材料，这是可供授权用户。
Chemotherapies feature a low success rate of about 25%, and therefore, the choice of the most effective cytostatic drug for the individual patient and monitoring the efficiency of an ongoing chemotherapy are important steps towards personalized therapy. Thereby, an objective method able to differentiate between treated and untreated cancer cells would be essential. In this study, we provide molecular insights into Docetaxel-induced effects in MCF-7 cells, as a model system for adenocarcinoma, by means of Raman microspectroscopy combined with powerful chemometric methods. The analysis of the Raman data is divided into two steps. In the first part, the morphology of cell organelles, e.g. the cell nucleus has been visualized by analysing the Raman spectra with k-means cluster analysis and artificial neural networks and compared to the histopathologic gold standard method hematoxylin and eosin staining. This comparison showed that Raman microscopy is capable of displaying the cell morphology; however, this is in contrast to hematoxylin and eosin staining label free and can therefore be applied potentially in vivo. Because Docetaxel is a drug acting within the cell nucleus, Raman spectra originating from the cell nucleus region were further investigated in a next step. Thereby we were able to differentiate treated from untreated MCF-7 cells and to quantify the cell–drug response by utilizing linear discriminant analysis models. Raman microspectroscopy in combination with powerful chemometric methods (e.g. artificial neural networks) indicates morphological (nucleus fragmentation) and spectral changes in Docetaxel treated breast cancer cells (MCF-7) in comparison to untreated cell samples The online version of this article (doi:10.1007/s00216-012-5887-9) contains supplementary material, which is available to authorized users.
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影响因子：
2.5
作者：
Cialla, Dana;Deckert-Gaudig, Tanja;Popp, Juergen
通讯作者：
Popp, Juergen
影响因子：
8
作者：
Hernandez-Vargas, H.;Palacios, J.;Moreno-Bueno, G.
通讯作者：
Moreno-Bueno, G.
影响因子：
2.9
作者：
Gaus, K.;Roesch, P.;Popp, J.
通讯作者：
Popp, J.
影响因子：
4.3
作者：
Hernandez-Vargas, Hector;Palacios, Jose;Moreno-Bueno, Gema
通讯作者：
Moreno-Bueno, Gema
影响因子：
4.2
作者：
Hermelink, Antje;Brauer, Angelika;Naumann, Dieter
通讯作者：
Naumann, Dieter