Multifunctional nanorods serving as nanobridges to modulate T cell-mediated immunity.
Multifunctional nanorods serving as nanobridges to modulate T cell-mediated immunity.
复制标题
DOI：
10.1021/nn403275p
复制
发表时间：
2013-11-26
期刊：
影响因子：
17.1
通讯作者：
Yoo, Hyuk Sang
中科院分区：
文献类型：
--
作者：
Son, Young Ju;Kim, Hyesung;Leong, Kam W.;Yoo, Hyuk Sang
Electrodeposited nanorods serving as multivalent bridges were fabricated and surface-decorated with ligands for immune cells. Gold and nickel solutions were sequentially electrodeposited on nanoporous anodized disc templates and the template was dissolved to retrieve bisegmented nanorods with different lengths. Gold and nickel segmented nanorods were surface-immobilized with mannose and RGD peptides to prepare immune-cell recruiting nanorods. Surface-functionalization of nanorods were confirmed by fluorescence-labeling of each ligands and confocal microscopy. Dendritic cells and T cells were co-incubated with the surface-functionalized nanorods, and the proximity between the nanorods and the immune cells was visualized by variable pressure scanning electron microscopy and confocal microscopy. The long nanorods were associated with the immune cells, whereas the shorter nanorods were rather endocytosed by cells, suggesting a feasibility of the longer nanorods as bridging for the cells. Cytokine releases from the immune cells were monitored by cultivating lipopolysaccharide-activated dendritic cells with T cells. Interleukine-2 and interferon-γ release profiles showed a strong correlation with the length of the nanorod, where the 4 μm nanorods induced the highest levels of cytokine release compared to 1 or 2 μm nanorods. Thus, we concluded that the proximity of the immune cells increased by bridging the immune cells with the nanobridging system, which subsequently increased cytokine release by facilitating the antigen presentation process.
登录
查看更多内容
影响因子：
13.3
作者：
Dreaden, Erik C.;Mwakwari, Sandra C.;Austin, Lauren A.;Kieffer, Matthew J.;Oyelere, Adegboyega K.;El-Sayed, Mostafa A.
通讯作者：
El-Sayed, Mostafa A.
影响因子：
17.4
作者：
Park, Shinyoung;Son, Young Ju;Yoo, Hyuk Sang
通讯作者：
Yoo, Hyuk Sang
影响因子：
5.4
作者：
Sheng, Kuo-Ching;Kalkanidis, Martha;Pietersz, Geoffrey A.
通讯作者：
Pietersz, Geoffrey A.
DOI：
10.1016/j.jconrel.2005.02.027
发表时间：
2005-07-20
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Kwon YJ;Standley SM;Goh SL;Fréchet JM
通讯作者：
Fréchet JM
金纳米颗粒的几何形状和表面特征会影响其生物分布和巨噬细胞的吸收。
DOI：
10.1016/j.ejpb.2010.11.010
发表时间：
2011-04
期刊：
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
影响因子：
--
作者：
Arnida;Janát-Amsbury MM;Ray A;Peterson CM;Ghandehari H
通讯作者：
Ghandehari H