Nano-evolution and protein-based enzymatic evolution predicts novel types of natural product nanozymes of traditional Chinese medicine: cases of herbzymes of Taishan-Huangjing (Rhizoma polygonati) and Goji (Lycium chinense).
Nano-evolution and protein-based enzymatic evolution predicts novel types of natural product nanozymes of traditional Chinese medicine: cases of herbzymes of Taishan-Huangjing (Rhizoma polygonati) and Goji (Lycium chinense).
复制标题
DOI：
10.1039/d1na00475a
复制
发表时间：
2021-11-24
影响因子：
4.7
通讯作者：
中科院分区：
文献类型：
--
作者：
纳米酶和天然产物衍生的草本酶已被鉴定为模拟天然蛋白质基酶功能的不同类型的酶。如何探索和预测合成时的新型纳米酶的酶类型仍然是难以捉摸的。有根据的分析可能对预测有用。在这里，我们应用蛋白质进化分析方法来预测新类型的酶与实验验证。首先，对已报道的纳米酶进行了化学分类和纳米进化分析。我们发现纳米酶主要被分类为基于蛋白质的EC 1氧化还原酶。比较而言，我们利用系统树分析了蛋白质基天然酶的进化，发现最保守的酶是过氧化物酶和裂解酶。因此，分析了黄芪和枸杞草药的天然产物，以使用天然蛋白酶进化的简单模拟来探索和测试有效的新型天然纳米酶/草药酶，因为它们包含这些保守的酶类型。实验验证表明，纳米级泰山黄精总提取物具有果糖二磷酸醛缩酶裂解酶活性，纳米级枸杞提取物具有过氧化物酶活性。因此，生物信息学分析将为天然产物纳米酶的虚拟发现提供额外的工具。纳米酶和天然产物衍生的草本酶已被鉴定为模拟天然蛋白质基酶功能的不同类型的酶。
Nanozymes and natural product-derived herbzymes have been identified in different types of enzymes simulating the natural protein-based enzyme function. How to explore and predict enzyme types of novel nanozymes when synthesized remains elusive. An informed analysis might be useful for the prediction. Here, we applied a protein-evolution analysis method to predict novel types of enzymes with experimental validation. First, reported nanozymes were analyzed by chemical classification and nano-evolution. We found that nanozymes are predominantly classified as protein-based EC1 oxidoreductase. In comparison, we analyzed the evolution of protein-based natural enzymes by a phylogenetic tree and the most conserved enzymes were found to be peroxidase and lyase. Therefore, the natural products of Rhizoma polygonati and Goji herbs were analyzed to explore and test the potent new types of natural nanozymes/herbzymes using the simplicity simulation of natural protein enzyme evolution as they contain these conserved enzyme types. The experimental validation showed that the natural products from the total extract of nanoscale traditional Chinese medicine Huangjing (RP, Rhizoma polygonati) from Mount-Tai (Taishan) exhibit fructose-bisphosphate aldolase of lyase while nanoscale Goji (Lycium chinense) extract exhibits peroxidase activities. Thus, the bioinformatics analysis would provide an additional tool for the virtual discovery of natural product nanozymes. Nanozymes and natural product-derived herbzymes have been identified in different types of enzymes simulating the natural protein-based enzyme function.
登录
查看更多内容
影响因子：
6.4
作者：
Robinson PK
通讯作者：
Robinson PK
影响因子：
4.1
作者：
Thomson, GJ;Howlett, GJ;Berry, A
通讯作者：
Berry, A
影响因子：
8
作者：
Xie, Yingqiu;Fan, Haiyan;Benassi, Enrico
通讯作者：
Benassi, Enrico
DOI：
10.1016/j.ijbiomac.2019.03.013
发表时间：
2019-06-01
影响因子：
8.2
作者：
Pawlaczyk-Graja, Izabela;Balicki, Sebastian;Wilk, Kazimiera A.
通讯作者：
Wilk, Kazimiera A.
影响因子：
3.9
作者：
Lorentzen, E;Siebers, B;Pohl, E
通讯作者：
Pohl, E