Cyclin C and cyclin dependent kinases 1, 2 and 3 in thrombin-induced neuronal cell cycle progression and apoptosis.
Cyclin C and cyclin dependent kinases 1, 2 and 3 in thrombin-induced neuronal cell cycle progression and apoptosis.
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DOI：
10.1016/j.neulet.2008.12.018
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发表时间：
2009-02-06
影响因子：
2.5
通讯作者：
Grammas P
中科院分区：
文献类型：
--
作者：
Rao HV;Thirumangalakudi L;Grammas P
神经元进入细胞周期的程度和细胞周期再进入导致细胞凋亡的机制随激动剂的反应而变化。我们之前表明，经凝血酶处理的神经元中早期G1调节因子上调，但神经元并未进入S期，而是发生凋亡。本研究的目的是探讨凝血酶处理神经元后阻止S期进入和促进凋亡的机制。将培养的大鼠脑神经元暴露于凝血酶（200 nM）30分钟至4.5小时，并评估细胞周期蛋白C、细胞周期蛋白依赖性激酶（cdk 1、2、3、8）和细胞周期抑制剂p27的表达。我们的数据显示，凝血酶治疗后不久，细胞周期蛋白C和cdk 3蛋白的同时减少。在mRNA和蛋白水平上，细胞周期蛋白C的减少也与cdk 1和cdk 2的减少相关。凝血酶处理后cdk 8或细胞周期抑制剂p27的表达没有变化。这些结果表明，G1-S期调节因子cyclin C和cdks 3，cdk 2和cdk 1在凝血酶反应中的减少可能使条件不利于S期进入并有利于神经元凋亡。
The extent to which neurons proceed into the cell cycle and the mechanisms whereby cell cycle re-entry leads to apoptosis vary in response to agonists. We previously showed upregulation of early G1 regulators in thrombin-treated neurons yet neurons did not proceed to S phase but to apoptosis. The objective of this study is to explore mechanisms which might prevent S phase entry and promote apoptosis in thrombin-treated neurons. Cultured rat brain neurons are exposed to thrombin (200 nM) for 30 min to 4.5 h and the expression of cyclin C, cyclin dependent kinases (cdk1, 2 ,3, 8) and the cell cycle inhibitor p27 assessed. Our data show a simultaneous decrease of both cyclin C and cdk3 proteins soon after thrombin treatment. The decrease in cyclin C also correlates with decreases in cdk1 and cdk2, at both mRNA and protein levels. There is no change in expression of cdk8 or the cell cycle inhibitor p27 in response to thrombin treatment. These results suggest that decreases in G1-S regulators cyclin C and cdks 3, cdk2 and cdk1 in response to thrombin could make conditions unfavorable for S phase entry and favor neuronal apoptosis.
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影响因子：
2.2
作者：
MCGEER, PL;KLEGERIS, A;MCGEER, EG
通讯作者：
MCGEER, EG
影响因子：
7.5
作者：
ELLEDGE, SJ;HARPER, JW
通讯作者：
HARPER, JW
影响因子：
64.5
作者：
Ren, SJ;Rollins, BJ
通讯作者：
Rollins, BJ
DOI：
10.1097/00004647-200202000-00005
发表时间：
2002-02-01
影响因子：
6.3
作者：
Wang, FH;Corbett, D;Park, DS
通讯作者：
Park, DS
影响因子：
2.5
作者：
AKIYAMA, H;IKEDA, K;MCGEER, PL
通讯作者：
MCGEER, PL