RNA-Seq De Novo Assembly of Red Pitaya (Hylocereus polyrhizus) Roots and Differential Transcriptome Analysis in Response to Salt Stress
RNA-Seq De Novo Assembly of Red Pitaya (Hylocereus polyrhizus) Roots and Differential Transcriptome Analysis in Response to Salt Stress
复制标题
红火龙果根的 RNA 测序从头组装和盐胁迫响应的差异转录组分析
DOI：
10.1007/s12042-019-09217-3
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发表时间：
2019-06
影响因子：
2
通讯作者：
Xia Kuaifei
中科院分区：
文献类型：
--
作者：
Nong Qu;ong;Zhang Mingyong;Chen Jiantong;Zhang Mei;Cheng Huaping;Jian Shuguang;Lu Hongfang;Xia Kuaifei
火龙果是一种价值很高的功能食品，在东南亚广泛种植。然而，随着种植区土壤盐渍化的加剧，更好地了解红火龙果对盐胁迫反应的分子机制是必要的。本研究利用RNA-Seq技术对火龙果红根在盐胁迫下的转录图谱进行了重新组装和鉴定。共获得73,589个转录本，平均序列长度为1308个碱基。从这些转录本中，26,878个独特的转录本成功地与13,519个Swiss-Prot蛋白质配对。基因本体论(GO)注释显示，在分子功能、生物功能和细胞组成类别中，催化活性(GO：0003824)、代谢过程(GO：0008152)和细胞部分(GO：0044464)分别是最丰富的。在暴露于450 mM氯化钠后的3个时间点(3小时、7小时和30小时)，2624个转录本的表达存在显著差异。此外，在所有三个时间点中，261个基因上调，61个基因下调。糖酵解/糖异生是最重要的调节途径之一。这些发现为火龙果的盐胁迫反应提供了进一步的洞察，并将为未来研究盐适应的功能研究提供有价值的资源。
Pitaya (Hylocereus polyrhizus) is a highly valued functional food that is widely planted in Southeast Asia. However, with increased soil salinization in cultivation areas, a better understanding of the molecular mechanisms associated with salt stress responses in red pitaya is necessary. Herein, RNA-Seq was used to de novo assemble and characterize the transcriptomic profiles of red pitaya roots in response to salt stress. A total of 73,589 transcripts were obtained and the average sequence length was 1308 bp. From these transcripts, 26,878 unique transcripts were successfully matched to 13,519 Swiss-prot proteins. Gene ontology (GO) annotations showed that within the categories of molecular function, biological function, and cell component, catalytic activity (GO:0003824), metabolic process (GO:0008152), and cell part (GO:0044464) were the most enriched, respectively. 2624 transcripts were significantly differentially expressed among three time points (3 h, 7 h, and 30 h) following exposure to 450 mM NaCl. Furthermore, 261 genes were up-regulated and 61 down-regulated in all three of the time points. Glycolysis/gluconeogenesis was one of the most significantly modulated pathways. The findings presented herein provide further insight into salt stress responses in pitaya and will provide a valuable resource for future functional studies examining salt adaptations.
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影响因子：
3.5
作者：
Qiong Nie;Guo-Li Gao;Qing-jie Fan;G. Qiao;X. Wen;Tao Liu;Zhijun Peng;Yongqiang Cai
通讯作者：
Qiong Nie;Guo-Li Gao;Qing-jie Fan;G. Qiao;X. Wen;Tao Liu;Zhijun Peng;Yongqiang Cai
影响因子：
2
作者：
Radwan, Osman;Arro, Jie;Korban, Schuyler S.
通讯作者：
Korban, Schuyler S.
DOI：
--
发表时间：
1995
期刊：
--
影响因子：
--
作者：
F. Ghassemi;A. Jakeman;H. Nix
通讯作者：
F. Ghassemi;A. Jakeman;H. Nix
影响因子：
2.8
作者：
de Carvalho, Kenia;Freitas de Campos, Marilia Kaphan;Esteves Vieira, Luiz Gonzaga
通讯作者：
Esteves Vieira, Luiz Gonzaga
影响因子：
6.2
作者：
Fan, Wei;Zhang, Zhili;Zhang, Yanlin
通讯作者：
Zhang, Yanlin