Micro-CT imaging of Onchocerca infection of Simulium damnosum s.l. blackflies and comparison of the peritrophic membrane thickness of forest and savannah flies.
Micro-CT imaging of Onchocerca infection of Simulium damnosum s.l. blackflies and comparison of the peritrophic membrane thickness of forest and savannah flies.
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DOI：
10.1111/mve.12509
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发表时间：
2021-09
影响因子：
1.9
通讯作者：
Cheke RA
中科院分区：
文献类型：
--
作者：
Hall MJR;Martín-Vega D;Clark B;Ghosh D;Rogers M;Pigoli D;Veriegh FBD;Tetteh-Kumah A;Osei-Atweneboana MY;Cheke RA
盘尾丝虫病是一种被忽视的热带疾病，由盘尾丝虫Diesing 1841(螺旋纲：盘尾丝虫科)线虫通过蝇传播引起。它与贫困有关，并给许多热带国家带来了巨大的健康、福利和经济负担。目前可视化载体内感染的方法依赖于侵入性方法。然而，使用微型计算机断层扫描技术，在没有物理组织操作干扰的情况下，我们第一次在黑蝇(Simulium Damnosum s.l)的胸肌中三维可视化了一种钉螺的L1幼虫。Theobald 1903(双翅目：姬蜂科)，在加纳自然感染。不支持草原果蝇较厚的围食膜可以解释它们较低的寄生虫量的可能性，但我们的分析存在局限性。在血餐后34-48 ，森林黑蝇和草原黑蝇的前部、背部和腹部的平均围食膜厚度差异无统计学意义，但不能测量后部的围食膜厚度。微型计算机断层扫描有可能为公众参与健康教育提供许多其他寄生虫/媒介系统的新信息和有影响力的图像。使用微型计算机断层扫描技术，在加纳自然感染的黑蝇Simulium Damnosum s.l的胸肌内，对一种钉螺的L1幼虫进行了3D可视化。森林黑蝇和草原黑蝇的平均围食膜厚度在血餐后34-48 之间差异无统计学意义。微型计算机断层扫描有可能为公众参与健康教育提供许多其他寄生虫/媒介系统的新信息和有影响力的图像。
Onchocerciasis is a neglected tropical disease (NTD) caused by Onchocerca Diesing 1841 (Spirurida: Onchocercidae) nematodes transmitted by blackflies. It is associated with poverty and imposes a significant health, welfare and economic burden on many tropical countries. Current methods to visualize infections within the vectors rely on invasive methods. However, using micro‐computed tomography techniques, without interference from physical tissue manipulation, we visualized in three dimensions for the first time an L1 larva of an Onchocerca species within the thoracic musculature of a blackfly, Simulium damnosum s.l. Theobald 1903 (Diptera: Simuliidae), naturally infected in Ghana. The possibility that thicker peritrophic membranes in savannah flies could account for their lower parasite loads was not supported, but there were limits to our analysis. While there were no statistically significant differences between the mean thicknesses of the peritrophic membranes, in the anterior, dorsal and ventral regions, of forest and savannah blackflies killed 34–48 min after a blood‐meal, the thickness of the peritrophic membrane in the posterior region could not be measured. Micro‐computed tomography has the potential to provide novel information on many other parasite/vector systems and impactful images for public engagement in health education. Micro‐computed tomography was used to visualize in 3D an L1 larva of an Onchocerca species within the thoracic musculature of a blackfly, Simulium damnosum s.l., naturally infected in Ghana. There were no statistically significant differences between the mean thicknesses of the peritrophic membranes of forest and savannah blackflies killed 34–48 min after a blood‐meal. Micro‐computed tomography has the potential to provide novel information on many other parasite/vector systems and impactful images for public engagement in health education.
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影响因子：
2.4
作者：
O'Sullivan JDB;Behnsen J;Starborg T;MacDonald AS;Phythian-Adams AT;Else KJ;Cruickshank SM;Withers PJ
通讯作者：
Withers PJ
影响因子：
4.6
作者：
Martín-Vega D;Garbout A;Ahmed F;Wicklein M;Goater CP;Colwell DD;Hall MJR
通讯作者：
Hall MJR
影响因子：
1.6
作者：
LAURENCE B R
通讯作者：
LAURENCE B R
影响因子：
1.9
作者：
LEWIS, DJ
通讯作者：
LEWIS, DJ
影响因子：
2.7
作者：
Cheke, Robert A.;Garms, Rolf
通讯作者：
Garms, Rolf