3D virtual histology at the host/parasite interface: visualisation of the master manipulator, Dicrocoelium dendriticum, in the brain of its ant host.
3D virtual histology at the host/parasite interface: visualisation of the master manipulator, Dicrocoelium dendriticum, in the brain of its ant host.
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DOI：
10.1038/s41598-018-26977-2
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发表时间：
2018-06-05
影响因子：
4.6
通讯作者：
Hall MJR
中科院分区：
文献类型：
--
作者：
Martín-Vega D;Garbout A;Ahmed F;Wicklein M;Goater CP;Colwell DD;Hall MJR
有些寄生虫能够操纵宿主的行为，使之对自己有利。关于宿主操纵的最完善的教科书例子之一是树突吸虫在蚂蚁上的例子，蚂蚁是它的第二个中间宿主。受感染的蚂蚁在胃内有囊性囊蚴，在食管下神经节（SOG）有非囊性囊蚴；然而，树突虫用来操纵蚂蚁行为的机制仍然未知，部分原因是缺乏对寄生虫和蚂蚁脑组织之间物理界面的适当和直接的可视化。在这里，我们通过使用非侵入性微ct扫描表征树突d和蚂蚁组织之间的界面，为这种标志性的操纵器用来改变宿主行为的潜在机制提供了新的见解。我们首次证明，在SOG的最前部，寄生虫与蚂蚁脑组织之间存在物理接触，包括在多重脑感染的情况下，只有位于SOG最前部的寄生虫与蚂蚁脑组织接触。我们展示了微型ct在寄生虫学研究中进一步了解其他寄生虫/宿主系统的潜力。
Some parasites are able to manipulate the behaviour of their hosts to their own advantage. One of the most well-established textbook examples of host manipulation is that of the trematode Dicrocoelium dendriticum on ants, its second intermediate host. Infected ants harbour encysted metacercariae in the gaster and a non-encysted metacercaria in the suboesophageal ganglion (SOG); however, the mechanisms that D. dendriticum uses to manipulate the ant behaviour remain unknown, partly because of a lack of a proper and direct visualisation of the physical interface between the parasite and the ant brain tissue. Here we provide new insights into the potential mechanisms that this iconic manipulator uses to alter its host’s behaviour by characterising the interface between D. dendriticum and the ant tissues with the use of non-invasive micro-CT scanning. For the first time, we show that there is a physical contact between the parasite and the ant brain tissue at the anteriormost part of the SOG, including in a case of multiple brain infection where only the parasite lodged in the most anterior part of the SOG was in contact with the ant brain tissue. We demonstrate the potential of micro-CT to further understand other parasite/host systems in parasitological research.
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影响因子：
2.4
作者：
O'Sullivan JDB;Behnsen J;Starborg T;MacDonald AS;Phythian-Adams AT;Else KJ;Cruickshank SM;Withers PJ
通讯作者：
Withers PJ
影响因子：
2.5
作者：
Dacks, AM;Christensen, TA;Hildebrand, JG
通讯作者：
Hildebrand, JG
影响因子：
2
作者：
Noever, Christoph;Keiler, Jonas;Glenner, Henrik
通讯作者：
Glenner, Henrik
影响因子：
4
作者：
van Paridon, Bradley J.;Colwell, Douglas D.;Gilleard, John S.
通讯作者：
Gilleard, John S.
影响因子：
1.3
作者：
Goater, Cameron P.;Colwell, Douglas D.
通讯作者：
Colwell, Douglas D.