Differential expression of mouse neural cell-adhesion molecule (N-CAM) mRNA species during brain development and in neural cell lines-猫眼课题宝

小鼠神经细胞粘附分子 (N-CAM) mRNA 种类在大脑发育过程中和神经细胞系中的差异表达

DOI：

发表时间：

1986

期刊：

Journal of Neuroscience

影响因子：

5.3

通讯作者：

中科院分区：

医学1区

文献类型：

作者：

G. Gennarini;MR Hirsch;H. He;M. Hirn;J. Finne;C. Goridis

细胞黏附分子N-CAM是形成细胞-细胞键的配体，在神经个体发育过程中发挥着重要作用。它们以几种分子形式存在，在蛋白质和碳水化合物水平上有所不同。这些不同形式的表达的调控是一个重要的问题，它关系到细胞之间的粘附性相互作用在形态发生过程中是如何被调节的。在本研究中，我们使用N-CAM的cDNA克隆来研究同源mRNAs在小鼠和大鼠脑内以及在两个神经细胞系中的表达。将结果与不同N-CAM蛋白水平进行比较。我们做了以下观察。一组由5个大小类别的mRNAs组成的复杂集合--它们在表达上显示了发育、区域和细胞类型相关的差异--与我们的1个cdna探针杂交。虽然胚胎脑含有长度为7.4、6.7和4.3kb的N-CAM基因转录本，但出生后还会出现另外两个5.2kb和2.9kb的mRNAs。转化的星形细胞特征的脑细胞主要表达6.7、4.3和2.9kb的mRNAs，神经母细胞瘤主要表达7.4、6.7、4.3和2.9kb的mRNAs。在大脑发育过程中，N-CAM信息量有重要的数量变化，在出生前后达到高峰，表明N-CAM的合成在转录水平上受到控制。对N-CAM蛋白和信使核糖核酸水平的比较表明，MR 120,000 N-CAM蛋白(N-CAM120)的相对浓度与5.2kb的转录本之间存在显著的相关性。

The cell-adhesion molecules N-CAM (neural cell-adhesion molecule) are ligands in the formation of cell-cell bonds and have been shown to play important roles during neuro-ontogenesis. They exist in several molecular forms which differ at the protein and carbohydrate levels. The regulation of the expression of these different forms is an important issue that bears on such questions as to how adhesive interactions between cells are modulated during morphogenesis. In the present study we have used N-CAM cDNA clones to investigate the expression of the cognate mRNAs in the mouse and rat brain and in 2 neural cell lines. The results were compared with the levels of the different N-CAM proteins. We made the following observations. A complex set of 5 size classes of mRNAs--which show developmental, regional, and cell-type-dependent variations in their expression--hybridize to 1 of our cDNA probes. While embryonic brain contains N-CAM gene transcripts 7.4, 6.7, and 4.3 kilobases (kb) in length, 2 additional mRNAs of 5.2 and 2.9 kb appear postnatally. Transformed brain cells of an astrocytic character express predominantly mRNAs of 6.7, 4.3, and 2.9 kb and a neuroblastoma line those of 7.4, 6.7, 4.3, and 2.9 kb. There are important quantitative changes in the amount of N-CAM message expressed during brain development, with a peak around birth, suggesting that N- CAM synthesis is controlled at the transcriptional level. A comparison of N-CAM protein and mRNA levels reveals a striking correlation between the relative concentrations of the Mr 120,000 N-CAM protein (N-CAM120) and the 5.2 kb transcript.(ABSTRACT TRUNCATED AT 250 WORDS)