Structural basis for the interaction of protein S1 with the Escherichia coli ribosome.
Structural basis for the interaction of protein S1 with the Escherichia coli ribosome.
复制标题
DOI：
10.1093/nar/gku1314
复制
发表时间：
2015-01
影响因子：
14.9
通讯作者：
Moll I
中科院分区：
文献类型：
--
作者：
Byrgazov K;Grishkovskaya I;Arenz S;Coudevylle N;Temmel H;Wilson DN;Djinovic-Carugo K;Moll I
在革兰氏阴性细菌中，多结构域蛋白S1对于翻译启动是必不可少的，因为它招募了mRNA，并促进了它在解码中心的定位。与其功能重要性形成鲜明对比的是，S1仍然缺乏大肠杆菌和嗜热杆菌核糖体的高分辨结构，因此控制S1-核糖体相互作用的分子机制仍然不清楚。在这里，我们用核磁共振、X射线结晶学和冷冻电子显微镜相结合的方法，研究了N-末端S1结构域D1在原子分辨率下与核糖体结合的结构。结合生化分析，结构表明S1主要通过稳定的π堆积作用与核糖体结合，在短的但保守的N末端片段中灵活地连接到结构域D1。这种相互作用被涉及蛋白质S2的锌结合口袋的盐桥进一步稳定。总体而言，这项工作提供了迄今为止“核糖体之谜”中的一块谜团，即对S1-核糖体界面拓扑的详细分子洞察。此外，我们的数据表明，新的机制有可能通过改变S1与核糖体的亲和力来调节蛋白质合成，以响应环境提示。
In Gram-negative bacteria, the multi-domain protein S1 is essential for translation initiation, as it recruits the mRNA and facilitates its localization in the decoding centre. In sharp contrast to its functional importance, S1 is still lacking from the high-resolution structures available for Escherichia coli and Thermus thermophilus ribosomes and thus the molecular mechanism governing the S1–ribosome interaction has still remained elusive. Here, we present the structure of the N-terminal S1 domain D1 when bound to the ribosome at atomic resolution by using a combination of NMR, X-ray crystallography and cryo-electron microscopy. Together with biochemical assays, the structure reveals that S1 is anchored to the ribosome primarily via a stabilizing π-stacking interaction within the short but conserved N-terminal segment that is flexibly connected to domain D1. This interaction is further stabilized by salt bridges involving the zinc binding pocket of protein S2. Overall, this work provides one hitherto enigmatic piece in the ′ribosome puzzle′, namely the detailed molecular insight into the topology of the S1–ribosome interface. Moreover, our data suggest novel mechanisms that have the potential to modulate protein synthesis in response to environmental cues by changing the affinity of S1 for the ribosome.
登录
查看更多内容
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
3
作者：
Chen, James Z.;Grigorieff, Nikolaus
通讯作者：
Grigorieff, Nikolaus
影响因子：
0.9
作者：
Giraud, Pierre;Crechet, Jean-Bernard;Sizun, Christina
通讯作者：
Sizun, Christina
DOI：
10.1111/j.1432-1033.1975.tb02202.x
发表时间：
1975-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
ISONO, S;ISONO, K
通讯作者：
ISONO, K
影响因子：
14.9
作者：
Delvillani, Francesco;Papiani, Giulia;Briani, Federica
通讯作者：
Briani, Federica