Effects of gelling bath on the physical properties of alginate gel beads and the biological characteristics of entrapped HepG2 cells
Effects of gelling bath on the physical properties of alginate gel beads and the biological characteristics of entrapped HepG2 cells
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凝胶浴对海藻酸盐凝胶珠物理性质及包埋HepG2细胞生物学特性的影响
DOI：
10.1002/bab.1585
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发表时间：
2018-03
期刊：
影响因子：
--
通讯作者：
Sun G
中科院分区：
文献类型：
--
作者：
un D;Liu Y;Wu H;Ren Y;Ma X;Wu H;Sun G
优化藻酸盐凝胶珠对于支持包埋肝细胞的存活、增殖和功能是必要的。在这项研究中，通过降低钙离子浓度和增加钠离子浓度来修改胶凝浴。与传统的仅含钙离子的凝胶浴相比，在改进的凝胶浴中制备的海藻酸钠凝胶珠（使用36%G海藻酸钠）具有更均匀的结构和更好的传质性能。此外，在改进的凝胶浴中制备的海藻酸钠凝胶珠能显著促进HepG 2细胞增殖和细胞球体的生长，并保持与仅含钙离子的传统凝胶浴中制备的海藻酸钠凝胶珠相似的白蛋白分泌能力。在改性凝胶浴中产生的具有不同M/G比（36%G和55%G）的ALG珠的传质性质和细胞增殖相似，而与传统凝胶浴中的海藻酸盐凝胶珠（55%G）相比，它们显著增加。结果表明，通过改变凝胶浴的方式可以提高藻酸盐凝胶珠的传质性能，为生物人工肝支持系统提供更多的肝细胞。
Optimizing alginate gel beads is necessary to support the survival, proliferation, and function of entrapped hepatocytes. In this study, gelling bath was modified by decreasing calcium ion concentration and increasing sodium ion concentration. Alginate gel beads (using 36% G sodium alginate) prepared in the modified gelling bath had more homogeneous structure and better mass transfer properties compared with the traditional gelling bath that contains only calcium ions. Moreover, alginate gel beads generated in the modified gelling bath could significantly promote the HepG2 cell proliferation and the growth of cell spheroids, and maintain the albumin secretion ability similar to alginate gel beads prepared in the traditional gelling bath with only calcium ions. The mass transfer properties and cell proliferation were similar in ALG beads with different M/G ratio (36% G and 55% G) generated in the modified gelling bath, whereas they were significantly increased compared with alginate gel beads (55% G) in traditional gelling bath. These results indicated that adjusting the gelling bath was a simple and convenient method to enhance the mass transfer properties of alginate gel beads for 3D hepatocyte culture, which might provide more hepatocytes for the bioartificial liver support system.
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