Fabrication of three-dimensional porous cell-laden hydrogel for tissue engineering.
Fabrication of three-dimensional porous cell-laden hydrogel for tissue engineering.
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DOI：
10.1088/1758-5082/2/3/035003
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发表时间：
2010-09
期刊：
影响因子：
9
通讯作者：
Khademhosseini A
中科院分区：
文献类型：
--
作者：
Hwang CM;Sant S;Masaeli M;Kachouie NN;Zamanian B;Lee SH;Khademhosseini A
在组织工程应用中，支架应该是多孔的，以便快速传递营养和氧气，同时为被封装的细胞提供三维（3D）微环境。这种双重特性可以通过制造包含被封装细胞的多孔水凝胶来实现。在这项工作中，我们开发了一种简单的方法，可以同时在海藻酸盐水凝胶内进行细胞包封和孔生成。用直径为150 ~ 300 μm的明胶珠作为牺牲孔隙剂，在含细胞的水凝胶中生成孔洞。明胶在低温（4°C）下凝胶化，形成无化学交联的珠粒，细胞包封后它们随后的溶解导致在细胞负载的水凝胶内产生孔。支架的孔径和孔隙率分别由明胶颗粒大小和它们的体积比控制。对制备的水凝胶进行了内部微结构、力学性能和渗透性表征。与无孔海藻酸盐水凝胶相比，随着明胶珠含量的增加，水凝胶表现出高度的孔隙度。随着孔隙率的增加，支架的渗透性增加2 ~ 3个数量级，而压缩模量降低。通过对肝癌细胞系（HepG2）的包封实验，验证了这些支架在组织工程中的应用。所有支架的细胞活力相似；然而，多孔条件下细胞增殖增强。此外，与非多孔条件相比，多孔藻酸盐水凝胶导致形成更大的球体和更高的白蛋白分泌。这些数据表明，多孔藻酸盐水凝胶可能为细胞增殖和白蛋白的产生提供了更好的环境。这可能是由于营养物质、氧气和废物清除的质量传递增强，这对组织工程和再生医学应用有潜在的好处。
For tissue engineering applications, scaffolds should be porous to enable rapid nutrient and oxygen transfer while providing a three-dimensional (3D) microenvironment for the encapsulated cells. This dual characteristic can be achieved by fabrication of porous hydrogels that contain encapsulated cells. In this work, we developed a simple method that allows cell encapsulation and pore generation inside alginate hydrogels simultaneously. Gelatin beads of 150–300 μm diameter were used as a sacrificial porogen for generating pores within cell-laden hydrogels. Gelation of gelatin at low temperature (4 °C) was used to form beads without chemical crosslinking and their subsequent dissolution after cell encapsulation led to generation of pores within cell-laden hydrogels. The pore size and porosity of the scaffolds were controlled by the gelatin bead size and their volume ratio, respectively. Fabricated hydrogels were characterized for their internal microarchitecture, mechanical properties and permeability. Hydrogels exhibited a high degree of porosity with increasing gelatin bead content in contrast to nonporous alginate hydrogel. Furthermore, permeability increased by two to three orders while compressive modulus decreased with increasing porosity of the scaffolds. Application of these scaffolds for tissue engineering was tested by encapsulation of hepatocarcinoma cell line (HepG2). All the scaffolds showed similar cell viability; however, cell proliferation was enhanced under porous conditions. Furthermore, porous alginate hydrogels resulted in formation of larger spheroids and higher albumin secretion compared to nonporous conditions. These data suggest that porous alginate hydrogels may have provided a better environment for cell proliferation and albumin production. This may be due to the enhanced mass transfer of nutrients, oxygen and waste removal, which is potentially beneficial for tissue engineering and regenerative medicine applications.
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影响因子：
14
作者：
Bryant, Stephanie J.;Cuy, Janet L.;Ratner, Buddy D.
通讯作者：
Ratner, Buddy D.
DOI：
10.1073/pnas.0801866105
发表时间：
2008-07-15
影响因子：
11.1
作者：
Du, Yanan;Lo, Edward;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
影响因子：
9.5
作者：
Hoch, G;Chauhan, A;Radke, CJ
通讯作者：
Radke, CJ
影响因子：
4.7
作者：
Boland, Thomas;Xu, Tao;Cui, Xiaofeng
通讯作者：
Cui, Xiaofeng
DOI：
10.1007/s10856-009-3818-x
发表时间：
2009-12-01
影响因子：
3.7
作者：
Haroun, Ahmed A.;Gamal-Eldeen, Amira;Harding, David R. K.
通讯作者：
Harding, David R. K.