Reciprocal regulation of NagC and quorum sensing systems and their roles in hmsHFRS expression and biofilm formation in Yersinia pseudotuberculosis.
Reciprocal regulation of NagC and quorum sensing systems and their roles in hmsHFRS expression and biofilm formation in Yersinia pseudotuberculosis.
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DOI：
10.1099/mic.0.001397
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发表时间：
2023-10
期刊：
影响因子：
2.8
通讯作者：
Atkinson, Steve
中科院分区：
文献类型：
--
作者：
Wiechmann, Anja;Garcia, Vanina;Elton, Linzy;Williams, Paul;Atkinson, Steve
生物膜形成 假结核耶尔森菌 受群体感应（QS）调节，并依赖于血红素储存位点hms，其是胞外多糖聚-N-乙酰葡糖胺（poly-GlcNAc）产生所需的。在 大肠杆菌 NagC调节GlcNAc生物合成和代谢，其中GlcNAc充当协调这些和其它活动的信号。然而，NagC和GlcNAc对生物膜发育的贡献在大肠杆菌中并不显著。 Y.假结核 是未知的。在这里，我们表明，一个Y。假结核nagC突变体在非生物（玻璃）和生物（秀丽隐杆线虫）表面上的生物膜产生受损。基因互补恢复了C.优雅的使用基于lux的启动子融合，发现hmsHFRS表达是nagC依赖的。鉴于NagC和QS都调节聚集和生物膜形成，我们使用基于lux的启动子融合研究了它们的调节关系。这些结果表明：（i）nagC是负性自调节的，但外源GlcNAc可以部分恢复nagC突变体中的表达，（ii）nagC负性调节ytbI和ypsI QS基因，（iii）nagC表达在ytbI、ypsI和ypsR突变体中降低，但在ytbR突变体中不降低。这些数据确立了NagC和QS之间存在相互调节关系，在luxRI对ytbRI的情况下，这也是GlcNAc依赖性的。因此，NagC和GlcNAc是涉及调节生物膜形成和聚集的QS的调节系统的组分。
Biofilm formation by Yersinia pseudotuberculosis is regulated by quorum sensing (QS) and dependent on the haemin storage locus hms, required for the extracellular polysaccharide poly-N-acetylglucosamine (poly-GlcNAc) production. In Escherichia coli NagC regulates both GlcNAc biosynthesis and metabolism with GlcNAc acting as a signal that co-ordinates these and other activities. However, the contribution of NagC and GlcNAc to biofilm development in Y. pseudotuberculosis is not known. Here we show that a Y. pseudotuberculosis nagC mutant is impaired for biofilm production on abiotic (glass) and biotic (Caenorhabitis elegans) surfaces. Genetic complementation restored poly-GlcNAc production and biofilm formation on C. elegans. Using lux-based promoter fusions, hmsHFRS expression was found to be nagC dependent. Given that NagC and QS both regulate aggregation and biofilm formation, we investigated their regulatory relationship using lux-based promoter fusions. These revealed that (i) nagC is negatively autoregulated, but expression can be partially restored in the nagC mutant by exogenous GlcNAc, (ii) NagC negatively regulates the ytbI and ypsI QS genes and (iii) nagC expression is reduced in the ytbI, ypsI and ypsR mutants but not the ytbR mutant. These data establish the existence of a reciprocal regulatory relationship between NagC and QS, which in the case of the luxRI pair ytbRI, is also GlcNAc-dependent. NagC and GlcNAc are therefore components of a regulatory system involving QS that modulates biofilm formation and aggregation.
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影响因子：
3.5
作者：
Ng YK;Grasso M;Wright V;Garcia V;Williams P;Atkinson S
通讯作者：
Atkinson S
影响因子：
3.6
作者：
Bobrov AG;Kirillina O;Ryjenkov DA;Waters CM;Price PA;Fetherston JD;Mack D;Goldman WE;Gomelsky M;Perry RD
通讯作者：
Perry RD
影响因子：
6.7
作者：
Atkinson S;Goldstone RJ;Joshua GW;Chang CY;Patrick HL;Cámara M;Wren BW;Williams P
通讯作者：
Williams P
影响因子：
3.6
作者：
Miyashiro T;Klein W;Oehlert D;Cao X;Schwartzman J;Ruby EG
通讯作者：
Ruby EG
影响因子：
3.1
作者：
Lavander, M;Ericsson, SK;Forsberg, Å
通讯作者：
Forsberg, Å