Biochemical reactions on a microfluidic chip based on a precise fluidic handling method at the nanoliter scale
Biochemical reactions on a microfluidic chip based on a precise fluidic handling method at the nanoliter scale
复制标题
基于纳升级精确流体处理方法的微流控芯片上的生化反应
DOI：
10.1007/bf02931899
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发表时间：
2006
影响因子：
3.2
通讯作者：
Byung
中科院分区：
文献类型：
--
作者：
Chang;Sang;Yun;Chang;Yong;Byung
设计了一种采用疏水阀和气动控制的被动微流控输送系统，用于芯片上的微流控处理。在芯片上正确地进行了两种液体的微流体计量、切割、输送和合并。在20 nL规模上，微流体计量的准确度的误差范围低于4%，这表明微流体易于在芯片上以所需体积进行操作。为了研究芯片上生化反应的可行性，进行单一酶促反应，例如β-半乳糖苷酶反应。底物，即β-半乳糖苷酶的荧光素二-β-吡喃半乳糖苷（FDG）（6.7 fM）的检测限为约76 pM。此外，在纳升尺度上成功地演示了多种生化反应，如增强型绿色荧光蛋白（EGFP）的体外蛋白质合成，这表明我们的微流控芯片不仅可以应用于各种生化反应的小型化，而且还可以用于开发需要精确的纳米尺度控制的微流控生化反应系统。
A passive microfluidic delivery system using hydrophobic valving and pneumatic control was devised for microfluidic handling on a chip. The microfluidic metering, cutting, transport, and merging of two liquids on the chip were correctly performed. The error range of the accuracy of microfluid metering was below 4% on a 20 nL scale, which showed that microfluid was easily manipulated with the desired volume on a chip. For a study of the feasibility of biochemical reactions on the chip, a single enzymatic reaction, such as a β-galactosidase reaction was performed. The detection limit of the substrate,i.e. fluorescein di-β-galactopyranoside (FDG) of the β-galactosidase (6.7 fM), was about 76 pM. Additionally, multiple biochemical reactions such asin vitro protein synthesis of enhanced green fluorescence protein (EGFP) were successfully demonstrated at the nanoliter scale, which suggests that our microfluidic chip can be applied not only to miniaturization of various biochemical reactions, but also to development of the microfluidic biochemical reaction system requiring a precise nano-scale control.
影响因子：
56.9
作者：
Unger, MA;Chou, HP;Quake, SR
通讯作者：
Quake, SR
影响因子：
7.4
作者：
Handique, K;Burke, DT;Burns, MA
通讯作者：
Burns, MA