Antibody‐Binding to the 180‐kD Bullous Pemphigoid Antigens at the Lateral Cell Surface Causes Their Internalization and Inhibits Their Assembly at the Basal Cell Surface in Cultured Keratinocytes
Antibody‐Binding to the 180‐kD Bullous Pemphigoid Antigens at the Lateral Cell Surface Causes Their Internalization and Inhibits Their Assembly at the Basal Cell Surface in Cultured Keratinocytes
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在培养的角质形成细胞中，抗体与外侧细胞表面的 180-kD 大疱性类天疱疮抗原结合导致其内化并抑制其在基底细胞表面的组装
DOI：
10.1111/j.1346-8138.1994.tb03299.x
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
H. Yaoita
中科院分区：
文献类型：
--
作者：
Y. Kitajima;Y. Hirako;K. Owaribe;S. Mori;H. Yaoita
我们通过免疫荧光显微镜证明了针对180 kD和230 kD BP抗原（BPA）的单克隆抗体和BP血清对培养的人角质形成细胞（细胞系，DJM-1）中Ca++诱导的半桥粒形成的影响。在低Ca++（0.07 mM）条件下，180-kD和230-kD BPA均匀分布在基底质膜上，而在高Ca++（1.87 mM）培养基中，它们形成了一个特殊的同心环或拱（环/拱）排列。另一方面，在低和高Ca++培养基中，顶侧细胞膜均被180 kD BPA抗体染色，但未被230 kD BPA抗体染色。低-高Ca++转换首先导致抗原从基底质膜消失，然后在3小时内形成高-Ca++环/弓模式。在该系统中，将针对180-kD和230-kD BPA的单克隆抗体以及来自5名BP患者、2名寻常型天疱疮（PV）患者和4名正常志愿者的血清添加到培养基中。添加抗180 kD BPA抗体或任何BP血清导致顶侧细胞膜中180 kD BPA内化，并抑制Ca++诱导的基底膜上环/弓图案的形成，可能是通过抑制抗原从侧膜向基底膜移动形成半桥粒。通过双重免疫染色显示内化的荧光点由180 kD BPA和患者的IgG组成，但不是由230 kD BPA组成，这表明内化的180 kD BPA来自顶侧膜，而不是来自半桥粒。抗230 kD BPA的单克隆抗体和正常及PV血清不会引起这些效应。这些结果表明，针对180 kD BPA而非230 kD BPA的自身抗体可能直接结合细胞表面上的抗原并干扰半桥粒的形成。
We demonstrated the effects of monoclonal antibodies to the 180‐kD and 230‐kD BP antigens (BPA) and of BP sera on Ca++‐induced formation of hemidesmosomes in cultured human keratinocytes (a cell line, DJM‐1) by immunofluorescence microscopy. Under low Ca++ (0.07 mM) conditions, the 180‐kD and 230‐kD BPAs were distributed homogeneously on the basal plasma membrane, while they formed a peculiar concentric ring or arch (ring/arch) arrangement in high‐Ca++ (1.87 mM) medium. On the other hand, the apical‐lateral cell membrane was stained homogeneously with antibodies to the 180‐kD BPA, but not to the 230‐kD BPA, both in low and high Ca++ media. The low‐high Ca++ switch at first caused disappearance of the antigen from the basal plasma membrane and then formed the high‐Ca++ ring/arch pattern within 3 hrs. In this system, monoclonal antibodies to the 180‐kD and 230‐kD BPAs and the sera from 5 BP patients, 2 pemphigus vulgaris (PV) patients, and 4 normal volunteers were added into the culture media. The addition of anti‐180‐kD BPA antibodies or any BP serum caused the internalization of the 180‐kD BPA from the apical‐lateral cell membrane and inhibited the Ca++‐induced formation of the ring/arch pattern on the basal membrane, possibly by inhibiting the movement of the antigen from the lateral to the basal membrane to form hemidesmosomes. The internalized fluorescence dots were shown to be composed of the 180‐kD BPA and patient's IgG, but not of the 230‐kD BPA by double‐immunostaining, suggesting the internalized 180‐kD BPA was from the apical‐lateral membrane, but not from hemidesmosomes. Monoclonal antibodies to the 230‐kD BPA and normal and PV sera did not cause these effects. These results suggest that autoantibodies to the 180‐kD BPA, but not to the 230‐kD BPA, may directly bind the antigen on the cell surface and disturb the formation of hemidesmosomes.
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DOI：
10.1016/s0141-8130(05)80004-2
发表时间：
1992-06-01
影响因子：
8.2
作者：
GREEN, KJ;VIRATA, MLA;PARRY, DAD
通讯作者：
PARRY, DAD
人大疱性类天疱疮抗原 (BPAG1)。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Sawamura,D;Li,K;Chu,ML;Uitto,J
通讯作者：
Uitto,J
通过亲和纯化的抗体鉴定两组大疱性类天疱疮抗原。
DOI：
10.1111/1523-1747.ep12876194
发表时间：
1990
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Meyer,LJ;Taylor,TB;Kadunce,DP;Zone,JJ
通讯作者：
Zone,JJ
影响因子：
4.4
作者：
Ramzy S. Labib;G. Anhalt;Harikrishna Patel;D. Mutasim;L. Diaz
通讯作者：
Ramzy S. Labib;G. Anhalt;Harikrishna Patel;D. Mutasim;L. Diaz
使用人类天疱疮自身抗体研究胰蛋白酶解离后表皮基底细胞半桥粒的命运。
DOI：
10.1111/1523-1747.ep12276893
发表时间：
1985
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Takahashi,Y;Mutasim,DF;Patel,HP;Anhalt,GJ;Labib,RS;Diaz,LA
通讯作者：
Diaz,LA