Impact of dietary organic acids and botanicals on intestinal integrity and inflammation in weaned pigs.
Impact of dietary organic acids and botanicals on intestinal integrity and inflammation in weaned pigs.
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DOI：
10.1186/s12917-015-0410-0
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发表时间：
2015-04-16
影响因子：
2.6
通讯作者：
Moeser AJ
中科院分区：
文献类型：
--
作者：
Grilli E;Tugnoli B;Passey JL;Stahl CH;Piva A;Moeser AJ
有机酸，如柠檬酸和山梨酸，以及纯植物来源的成分，如单萜和醛，在猪饲养中作为抗生素生长促进剂的替代品有很长的历史。然而，它们对肠屏障功能和炎症的影响从未被研究过。因此，本研究的目的是评估柠檬酸和山梨酸（OA）和纯植物药（即百里酚和香草醛）（PB）的微胶囊化混合物对断奶仔猪肠道完整性和功能性以及体外Caco-2细胞的影响。在第一项研究中，将20头仔猪分为2组，并在断奶后接受基础饮食或补充OA + PB（5g/kg）的基础饮食2周，在结束时收集回肠和空肠样品用于跨上皮电阻（TER）、间歇短路电流（ISC）和葡聚糖通量的Ussing室分析。还收集回肠粘膜的刮片用于细胞因子分析（n = 6）。在第二项研究中，我们直接测量了这些化合物对用0.2或1 g/l OA + PB处理的Caco-2单层的TER和渗透性的影响。饲喂OA + PB的猪回肠ISC倾向于降低（P = 0.07），并且IL-12、TGF-β和IL-6的回肠基因表达下调。在对Caco-2细胞的体外研究中，在实验的第4、6和14天补充0.2g/l增加TER，而在处理的第10和12天补充1g/l增加TER（P < 0.05）。葡聚糖通量没有受到显著影响，尽管在第7天和第14天观察到降低（分别为P = 0.10和P = 0.09）。总的来说，考虑到两个实验的结果，OA + PB通过调节局部和全身炎症压力改善了肠粘膜的成熟，最终导致肠道渗透性降低，并最终改善了过早断奶仔猪的生长。
Organic acids, such as citric and sorbic acid, and pure plant-derived constituents, like monoterpens and aldehydes, have a long history of use in pig feeding as alternatives to antibiotic growth promoters. However, their effects on the intestinal barrier function and inflammation have never been investigated. Therefore, aim of this study was to assess the impact of a microencapsulated mixture of citric acid and sorbic acid (OA) and pure botanicals, namely thymol and vanillin, (PB) on the intestinal integrity and functionality of weaned pigs and in vitro on Caco-2 cells. In the first study 20 piglets were divided in 2 groups and received either a basal diet or the basal diet supplemented with OA + PB (5 g/kg) for 2 weeks post-weaning at the end of which ileum and jejunum samples were collected for Ussing chambers analysis of trans-epithelial electrical resistance (TER), intermittent short-circuit current (ISC), and dextran flux. Scrapings of ileum mucosa were also collected for cytokine analysis (n = 6). In the second study we measured the effect of these compounds directly on TER and permeability of Caco-2 monolayers treated with either 0.2 or 1 g/l of OA + PB. Pigs fed with OA + PB tended to have reduced ISC in the ileum (P = 0.07) and the ileal gene expression of IL-12, TGF-β, and IL-6 was down regulated. In the in vitro study on Caco-2 cells, TER was increased by the supplementation of 0.2 g/l at 4, 6, and 14 days of the experiment, whereas 1 g/l increased TER at 10 and 12 days of treatment (P < 0.05). Dextran flux was not significantly affected though a decrease was observed at 7 and 14 days (P = 0.10 and P = 0.09, respectively). Overall, considering the results from both experiments, OA + PB improved the maturation of the intestinal mucosa by modulating the local and systemic inflammatory pressure ultimately resulting in a less permeable intestine, and eventually improving the growth of piglets prematurely weaned.
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DOI：
10.1152/ajpgi.00304.2006
发表时间：
2007-08-01
影响因子：
4.5
作者：
Moeser, Adam J.;Ryan, Kathleen A.;Blikslager, Anthony T.
通讯作者：
Blikslager, Anthony T.
影响因子：
5.9
作者：
Alexander, Lindsey S.;Seabolt, Brynn S.;Stahl, Chad H.
通讯作者：
Stahl, Chad H.
影响因子：
4.4
作者：
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关于单二烯的抗炎活性的综述。
DOI：
10.3390/molecules18011227
发表时间：
2013-01-18
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
de Cássia da Silveira e Sá R;Andrade LN;de Sousa DP
通讯作者：
de Sousa DP
影响因子：
4.2
作者：
Pié, S;Lallès, JP;Oswald, IP
通讯作者：
Oswald, IP