9-cis-epoxycarotenoid dioxygenase 1 confers heat stress tolerance in rice seedling plants.
9-cis-epoxycarotenoid dioxygenase 1 confers heat stress tolerance in rice seedling plants.
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9-顺式环氧类胡萝卜素双加氧酶1赋予水稻幼苗耐热胁迫能力
DOI：
10.3389/fpls.2022.1092630
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
高温是影响植物生长发育的主要制约因素之一。据报道，脱落酸（ABA）合成基因9-顺式环氧类胡萝卜素双加氧酶（NCED）可正向调节植物对盐、寒冷和干旱胁迫的抵抗力。然而，人们对NCED基因在水稻耐热性中的功能知之甚少。在这里，我们发现 OsNCED1 是一种热应激诱导基因。过表达 OsNCED1 的水稻幼苗表现出增强的耐热性，并且 ABA 含量更丰富，而敲除突变体 osnced1 积累的 ABA 更少，对热胁迫更敏感。在热胁迫下，OsNCED1表达增加可以减少植物膜损伤和活性氧（ROS）水平，并提高抗氧化酶的活性。此外，实时定量PCR（RT-qPCR）分析表明，OsNCED1的过度表达显着激活了抗氧化酶、ABA信号通路、热反应和防御相关基因的表达。总之，我们的结果表明，OsNCED1 通过提高内源 ABA 含量来正向调节水稻幼苗的耐热性，从而提高抗氧化能力并激活热和 ABA 相关基因的表达。
High temperature is one of the main constraints affecting plant growth and development. It has been reported that abscisic acid (ABA) synthesis gene 9-cis-epoxycarotenoid dioxygenase (NCED) positively regulates plant resistance to salt, cold, and drought stresses. However, little is known about the function of the NCED gene in heat tolerance of rice. Here, we found that OsNCED1 was a heat stress inducible gene. Rice seedlings overexpressing OsNCED1 showed enhanced heat tolerance with more abundant ABA content, whereas the knockout mutant osnced1 accumulated less ABA and showed more sensitive to heat stress. Under heat stress, increased expression of OsNCED1 could reduce membrane damage and reactive oxygen species (ROS) level of plants, and elevate the activity of antioxidant enzymes. Moreover, real time-quantitative PCR (RT-qPCR) analysis showed that overexpression of OsNCED1 significantly activated the expression of genes involved in antioxidant enzymes, ABA signaling pathway, heat response, and defense. Together, our results indicate that OsNCED1 positively regulates heat tolerance of rice seedling by raising endogenous ABA contents, which leads to the improved antioxidant capacity and activated expression of heat and ABA related genes.
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