Structural basis for 16S ribosomal RNA cleavage by the cytotoxic domain of colicin E3.
Structural basis for 16S ribosomal RNA cleavage by the cytotoxic domain of colicin E3.
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DOI：
10.1038/nsmb.1896
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发表时间：
2010-10
影响因子：
16.8
通讯作者：
中科院分区：
文献类型：
--
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The toxin colicin E3 targets the 30S subunit of bacterial ribosomes and cleaves a phosphodiester bond in the decoding center. We present the crystal structure of the 70S ribosome in complex with the cytotoxic domain of colicin E3 (E3-rRNase). The structure reveals how the rRNase domain of colicin binds to the A site of the decoding center in the 70S ribosome and cleaves 16S rRNA between A1493 and G1494. The cleavage mechanism involves the concerted action of conserved residues Glu62 and His58 of the cytotoxic domain of colicin E3 that activate the 16S rRNA for 2′ OH induced hydrolysis. Conformational changes observed for E3-rRNase, 16S rRNA and Helix 69 of 23S rRNA suggest that a dynamic binding platform is required for colicin E3 binding and function.
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影响因子：
64.5
作者：
Brodersen, DE;Clemons, WM;Ramakrishnan, V
通讯作者：
Ramakrishnan, V
DOI：
10.1107/s0907444998003254
发表时间：
1998-09-01
期刊：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
影响因子：
--
作者：
Brunger, AT;Adams, PD;Warren, GL
通讯作者：
Warren, GL
影响因子：
3.6
作者：
Bouveret, E;Rigal, A;Bénédetti, H
通讯作者：
Bénédetti, H
DOI：
10.1073/pnas.71.9.3380
发表时间：
1974-01-01
影响因子：
11.1
作者：
JAKES, KS;ZINDER, ND
通讯作者：
ZINDER, ND
影响因子：
3.9
作者：
James, R;Penfold, CN;Kleanthous, C
通讯作者：
Kleanthous, C