The secreted factor Ag1 missing in higher vertebrates regulates fins regeneration in Danio rerio.
The secreted factor Ag1 missing in higher vertebrates regulates fins regeneration in Danio rerio.
复制标题
较高脊椎动物中的分泌因子AG1缺少调节Danio Rerio的鳍的再生。
DOI：
10.1038/srep08123
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发表时间：
2015-01-29
影响因子：
4.6
通讯作者：
Zaraisky AG
中科院分区：
文献类型：
--
作者：
Ivanova AS;Shandarin IN;Ermakova GV;Minin AA;Tereshina MB;Zaraisky AG
Agr家族包括Ag1、Ag2和Ag3三组基因，它们编码含硫氧还蛋白结构域的分泌蛋白，最近被证明参与两栖动物截肢肢体的再生。相比之下，高等脊椎动物只有Ag2和Ag3，但缺乏Ag1，并且再生身体附属物的能力很低。因此，我们可以假设，在进化过程中Ag 1的丢失可能是导致高等脊椎动物再生能力下降的重要事件。为了验证这一点，我们现在已经研究了Ag 1的表达和作用，在再生的鳍的另一个大组的低等脊椎动物，鱼Danio rerio的代表。因此，我们已经证明，截肢的Danio鳍，像截肢的身体附属物在两栖动物，elephantan增加Ag 1表达的细胞的树桩。此外，下调DAg1体内吗啉代反义寡核苷酸注射导致鳍再生的延迟。这些数据很好地符合假设，即在高等脊椎动物祖先中Ag1的丢失可能导致其现代后代再生能力的降低。
Agr family includes three groups of genes, Ag1, Agr2 and Agr3, which encode the thioredoxin domain-containing secreted proteins and have been shown recently to participate in regeneration of the amputated body appendages in amphibians. By contrast, higher vertebrates have only Agr2 and Agr3, but lack Ag1, and have low ability to regenerate the body appendages. Thus, one may hypothesize that loss of Ag1 in evolution could be an important event that led to a decline of the regenerative capacity in higher vertebrates. To test this, we have studied now the expression and role of Ag1 in the regeneration of fins of a representative of another large group of lower vertebrates, the fish Danio rerio. As a result, we have demonstrated that amputation of the Danio fins, like amputation of the body appendages in amphibians, elicits an increase of Ag1 expression in cells of the stump. Furthermore, down-regulation of DAg1 by injections of Vivo-morpholino antisense oligonucleotides resulted in a retardation of the fin regeneration. These data are in a good agreement with the assumption that the loss of Ag1 in higher vertebrates ancestors could lead to the reduction of the regenerative capacity in their modern descendants.
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DOI：
10.1186/bcr2586
发表时间：
2010
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Vanderlaag KE;Hudak S;Bald L;Fayadat-Dilman L;Sathe M;Grein J;Janatpour MJ
通讯作者：
Janatpour MJ
影响因子：
56.9
作者：
Whitehead, GG;Makino, S;Keating, MT
通讯作者：
Keating, MT
DOI：
10.1073/pnas.1108472108
发表时间：
2011-08-16
影响因子：
11.1
作者：
Kumar, Anoop;Delgado, Jean-Paul;Brockes, Jeremy P.
通讯作者：
Brockes, Jeremy P.
影响因子：
1.2
作者：
Shih, Li-Jane;Lu, Yu-Fen;Hwang, Sheng-Ping L.
通讯作者：
Hwang, Sheng-Ping L.
影响因子：
2.4
作者：
Tereshina, Maria B.;Ermakova, Galina V.;Zaraisky, Andrey G.
通讯作者：
Zaraisky, Andrey G.