Exploring Antimicrobial Resistance Profiles of E. coli Isolates in Dairy Cattle: A Baseline Study across Dairy Farms with Varied Husbandry Practices in Puerto Rico.
Exploring Antimicrobial Resistance Profiles of E. coli Isolates in Dairy Cattle: A Baseline Study across Dairy Farms with Varied Husbandry Practices in Puerto Rico.
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DOI：
10.3390/microorganisms11122879
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发表时间：
2023-11-28
期刊：
影响因子：
4.5
通讯作者：
Dudley, Edward G.
中科院分区：
文献类型：
--
作者：
Malavez, Yadira;Nieves-Miranda, Sharon M.;Loperena Gonzalez, Paola N.;Padin-Lopez, Adrian F.;Xiaoli, Lingzi;Dudley, Edward G.
家畜抗菌素治疗可促进抗微生物药物耐药性（AMR）微生物的出现和传播。尽管在美国大陆对抗菌素耐药性细菌进行了大量监测，但这些抗菌素耐药性细菌在美国领土（如波多黎各）的流行程度仍未得到充分研究。本研究的目的包括获取波多黎各不同饲养方法的奶牛场分离的大肠杆菌抗微生物谱的基线数据。研究人员从两种传统奶牛场收集了79份粪便样本：一种是用罐奶喂养小牛，另一种是用废奶喂养小牛。这些样本是从动物的直肠、培养物中收集的，并随后通过生化测试加以证实。在这些样本中，对32个分离株进行了表型和基因表型分析，以阐明其AMR谱。结果强调了犊牛和成年牛之间抗微生物药物抗性基因发生的差异。值得注意的是，与罐奶喂养的小牛相比，废奶喂养的小牛表现出明显更高的耐抗生素大肠杆菌患病率。这些差异强调需要进行更全面的调查以确定致病因素。这些结果强调，迫切需要制定综合战略，提高人们对管理做法如何影响抗微生物药物耐药性的认识，将重点从治疗转向预防。
Antimicrobial treatment in livestock can contribute to the emergence and spread of antimicrobial-resistant (AMR) microorganisms. Despite substantial surveillance of AMR bacteria in the continental United States, the prevalence of these AMR organisms in U.S. territories, such as Puerto Rico, remains understudied. The goals of this research included obtaining baseline data on the antimicrobial profile of E. coli isolates from Puerto Rico dairy farms with different husbandry practices. Seventy-nine fecal samples were collected from two types of conventional dairy farms: those that fed calves with tank milk and those that fed calves with waste milk. These samples were collected from the animals’ rectums, culture, and subsequently confirmed through biochemical tests. Out of these samples, 32 isolates were analyzed phenotypically and genotypically to elucidate their AMR profiles. The results underscore a discrepancy in the occurrence of antimicrobial resistance genes between calves and adult cattle. Notably, waste milk-fed calves exhibited a significantly higher prevalence of antibiotic-resistant E. coli when compared to their tank milk-fed counterparts. These disparities emphasize the need for more comprehensive investigations to determine causative factors. These results underscore the urgency of comprehensive strategies to raise awareness about how management practices influence antimicrobial resistance, shifting the focus from treatment to prevention.
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DOI：
10.3390/antibiotics10080910
发表时间：
2021-07-26
期刊：
Antibiotics (Basel, Switzerland)
影响因子：
--
作者：
Eibl C;Bexiga R;Viora L;Guyot H;Félix J;Wilms J;Tichy A;Hund A
通讯作者：
Hund A
影响因子：
9.4
作者：
Joensen, Katrine Grimstrup;Scheutz, Flemming;Aarestrup, Frank M.
通讯作者：
Aarestrup, Frank M.
影响因子：
3.7
作者：
Belmar, Marlene;Aly, Sharif;Pereira, Richard, V
通讯作者：
Pereira, Richard, V
影响因子：
3.2
作者：
Edgar, R;Bibi, E
通讯作者：
Bibi, E
DOI：
10.1093/jac/dkaa345
发表时间：
2020-12-01
期刊：
The Journal of antimicrobial chemotherapy
影响因子：
--
作者：
Bortolaia V;Kaas RS;Ruppe E;Roberts MC;Schwarz S;Cattoir V;Philippon A;Allesoe RL;Rebelo AR;Florensa AF;Fagelhauer L;Chakraborty T;Neumann B;Werner G;Bender JK;Stingl K;Nguyen M;Coppens J;Xavier BB;Malhotra-Kumar S;Westh H;Pinholt M;Anjum MF;Duggett NA;Kempf I;Nykäsenoja S;Olkkola S;Wieczorek K;Amaro A;Clemente L;Mossong J;Losch S;Ragimbeau C;Lund O;Aarestrup FM
通讯作者：
Aarestrup FM